Inhibitive Effect Of Octreotide On Retinal Neovascularization

Objective:To explore inhibitive effect of octreotide on retinal neovascularization in the mouse.Method:Forty one-week-old mice were randomly divided into five groups that include normal group (Group N), high oxygen control group (Group O0), experimental group (GroupO1,O2,O3).The normal group was fed in the normal environment. High oxygen control group and experimental group were exposed to 80% oxygen for five days to establish models of retinal neovascularization, The normal group and high oxygen control group were received an intravitreal injection of 3μl of NS on postnatal day 12. While the experimental group were given in different concentration of 0.02μg,0.1μg,0.5μg octreotide in the vitreous 3μl. After 7 days, these mice were sacrificed. We can observed retinal blood vessel by the retinal flat and counted the number of the endotheliocyte nuclei of new blood vessels by retinal tissue-HE staining.Results:From retinal flat, there were uneven distribution of retinal blood vessels in the high oxygen contral group (O0) compared with in the normal group (N group),and the density and shape of retinal blood vessels were disordered. Distribution and morphology of retinal blood vessels gradually improved with the increases of the dose of octreotide in the experimental group (O1,O2,O3)).The number of the endotheliocyte nuclei of new blood vessels was (0.75±0.03) in Group N, (26.40±1.90) in Group O0),(19.37±1.24) in Group O1, (7.12±0.63) in Group 02,(2.38±0.16) in Group O3. Significant difference was found between Group N and Group Oo. There are alse showed significant difference between high oxygen control group and experimental group.Conclusion:Octreotide can inhibit retinal neovascularization in the mouse, and with the increase in dose inhibition become more obvious.