The Study On The Expression Of B Lymphocye And IL-12 In OLP

The prevalence of oral lichen planus (OLP) ranks second in oral mucosal diseases. Partial cases can transform from OLP into cancer. Accordingly, the World Health Organization(WHO) presume this disease a possible precancer. The etiology and pathogenesis are still obscure. There are extensive data to suggest that OLP is a T cell-mediated immune damage. Much effort has been spent on OLP therapy, but the outcome is still far from satisfaction. Histopathologic examination typically shows orthokeratotic hyperkeratosis, basal cell degeneration, and a dense well-defined infiltrate of lymphocytes in the superficial dermis. In OLP, there is a few B lymphocytes in the lymphocyte infiltration. CD20 is the surface marker of B cell especially. IL-12 is excreted by B cell, mononuclear cell, keratinocyte and DC. IL-12 play a unique role in promoting type 1 T helper cell responses and, thereby, cell-mediated immunity. IL-12 has been shown to exert striking therapeutic effects in a variety of infectious diseases.Objective:To detect the different expression of B cell, IL-12 in the tissue of normal oral mucosa and OLP at the level of protein by Immunohistochemistry and real time PCR respectively. And it is to be discussed about the function and mechanism involved with B cell and IL-12 in OLP. Different treatment means may be attempted.Methods:1. The study group consisted of 25 cases, included 20 cases of OLP and 5 cases with normal oral mucosa. These specimens were all defined by clinical and pathological diagnoses. Every specimen would be seriate cut for HE staining, Immunohistochemical staining of CD20 and IL-12p40, negative contrast,respectively. We studied the expression of CD20 and IL-12 in different tissues according to the results at the levels of protein by IH.2. OLP (n=6) and normal control (n=6) tissues were defined clinically and pathologically. To detect the expression of IL-12 using PCR technique and real-time PCR.3. To observe the distribution of CD20+B lymphocyte and IL-12 in OLP and normal oral tissue. To analyze the data of IL-12p40 mRNA using statistically system.Results:1. IH results of CD20:In normal mucosa, CD20 was negative.In OLP tissue, CD20 was positive in the lymphocytes infiltration.2. IH results of IL-12p40:In normal mucosa, IL-12p40 was positive in spinous layer cells, granulosa layer cells and connective tissues. In OLP, IL-12p40 had similar expression with normal mucosa in epithelium but with a stronger staining.3. Compared to normal oral mucosa, the expression of IL-12p40 mRNA is higher in OLP. Values of P<0.05 were considered statistically significant.Conclusion:CD20 in the tissue of OLP showed a strong expression, while none in the normal oral mucosa. IL-12p40 mRNA in the tissue of OLP shouwed a stronger expression than that in the tissue of normal oral mucosa. CD20+B cell may play a unique role in OLP development and progression by excreting IL-12.