Preliminary Study On Pathogenesis Of Oral Lichen Planus And Evaluation Of Precancerous Monitoring Means

Objective:The aim of the study was to analyze the level of intracellular Ca2+, which involved in lymphocyte activation. And analyze the expression of Golli-MBP (genes of the oligodendrocyte lineage) and stromal interaction molecule1(STIM1) in oral lichen planus (OLP), as they can regulate intracellular Ca2+.Then estimate their relationship with the pathogenesis of OLP.Methods:Ca2+fluorescent probe, Fluo-3/AM, was used to test the level of intracellular Ca2+in43patients with OLP and20healthy controls peripheral blood lymphocytes. The mRNA expression and protein levels of Golli-MBP and STIM1were analysed by quantitative real-time PCR (qRT-PCR) and western blot, respectively.Results:After lymphocyte activation, intracellular Ca2+in OLP patients were markly lower than control group (P<0.001).In OLP patients, the expression levels of Golli-MBP mRNA and protein were significantly upregulated when compared to that in the control group (P<0.001). With the same trend, OLP patients showed markedly upregulated levels of STIM1mRNA expression (P<0.01) and protein expression when compared with healthy controls.Conclusions:The intracellular Ca2+in OLP patients were markly lower than healthy controls, This may indicates that Ca2+signaling pathways in OLP patients may be abnormal.The overexpression of Golli-MBP and STIM1may play a role in the pathogenesis of OLP. Objective:Evaluating sensitivity and specificity of exfoliated cells DNA content analysis in monitoring oral potentially malignant disorders, to seek a more effective and noninvasive means in monitoring oral potentially malignant disorders.Methods:Exfoliative cytology (EC) was obtained using a cytobrush cell collector from52patients with potentially malignant disorders, followed by scalpel biopsy from the same region. Nuclear DNA contents (c) were measured after Feulgen restaining, using an automated DNA image cytometer. Exfoliative cytology with DNA-image cytometry and histopathological diagnosis were performed separately at different institutions.Results:In these cases, if abnormal DNA stemlines or cells with DNA content greater than5c were observed, they were assumed to indicate DNA aneuploidy. Histological investigation was considered the gold standard. The sensitivity of DNA aneuploidy for the detection of cancer cells in potentially malignant oral disorders was86.36%, its specificity was90.0%, false positive rate was13.64%, false negative rate was10.00%,its positive predictive value was86.36%, and its negative predictive value was90.00%.Conclusions:Brush biopsy with DNA-image cytometry is an accurate, noninvasive, easily practicable, economical screening method for diagnosing potentially malignant oral disorders.