Establishment Of Animal Model Of Oral Lichen Planus And The De-tection And Clinical Significance Of Salivary Enzymes, IL-18in Patients With Oral Lichen Planus

Oral lichen planus is one of the common diseases of oral mucosa, is a stubbornrefractory oral mucosal disease, its pathogenesis is not yet very clear.In treatment,scholars at home and abroad have already carried on a large number of basic andclinical research, but there has not been effective clinical treatment for this disease,the studies on oral lichen planus has not yet made a breakthrough. Investigate itsreason, it is important that has yet to establish an ideal animal model for its research.Therefore, for the establishment of animal models of oral lichen planus has beenbecoming a hot research topic.Oral mucosa is in an environment surrounded by saliva for a long time, andOLP as an oral mucosal disease, which is closely related with saliva. When OLPoccurs, it may cause changes of some components in saliva. Saliva has theadvantages of simple collection method,harmless to human body,easy to beaccepted by the patient and so on.Therefore,this study was detected the level ofALT,AST,LDH in saliva of OLP patients before and after treatment and healthypeople by automatic biochemical analyzer to explore the relationship between OLPand salivary enzymes, seeking OLP diagnostic laboratory evidence. Meanwhile, theanalysis of the expression of IL-18among normal oral mucosa tissue and OLPtissue by immunohistochemistry to determine whether IL-18is associated with theoccurrence of oral lichen planus.Part One: Establishment of animal model of Oral lichen planusObjective：Establish an animal model of oral lichen planus by local injectingand acupuncture scratching the tissue homogenate of OLP tissue to cheek pouchmucosa and dorsal skin of the golden hamster. Methods: Take a little lesion tissue of OLP patients,and make it into tissuehomogenate. Select10SPF level LVG golden hamsters, rats aged six weeks, bodyweight is about150g. They were randomly divided into5groups,2rats in eachgroup. The first group was the control group,the2nd and3rd groups were injectedthe tissue homogenate to the rats’ oral submucous and dorsal skin epidermis,the4thand5th groups were acupuncture scratched the tissue homogenate to the rats’ oralmucous and dorsal skin. The2nd and4th groups were killed after2weeks,drawnspecimen at oral mucosa and dorsal skin,the3rd and5th groups were killed after4weeks,drawn specimen at oral mucosa and dorsal skin.Make them into HE stainingto observe whether the pathological changes under light microscope.Results:The golden hamsters with tissue homogenate local injected,have nosignificant difference with the control group by visual inspection. Vascularhyperplasia and scattered inflammatory cell infiltration were observed in thefollowing deep skin appendages and oral submucosa under microscope. The goldenhamsters with tissue homogenate acupuncture scratched,which the experimental siteof skin color is darker than the normal control group, and has mild skin exfoliation,the oral mucosa is the same as the normal control group by visual inspection. Undermicroscope, skin epidermis layer visible epithelial cell hyperplasia andinflammatory cells infiltration under the skin epidermis, epithelial cell hyperplasiaand chronic inflammatory cell infiltration under the epithelium were showed at oralmucosa layer. And4weeks of inflammatory cells was significantly increasedcompared with2weeks.Conclusion:1Establish an animal model of oral lichen planus by local injecting andacupuncture scratching the tissue homogenate of OLP tissue to cheek pouch mucosaand dorsal skin of the golden hamster were failed，Only make the experimental sitein chronic inflammation pathological manifestations. For establishing the animalmodels of OLP still need further improvement.2Acupuncture scratch method of epithelial cell hyperplasia, inflammatory cellinfiltration occurred in oral mucosa and skin epidermis and the dermis, and thesepathologies are closer to the performans of OLP epithelial cell hyperplasia of prickle cell layer and lamina propria lymphocyte infiltration,its performance is better thanthat of local injections. Part Two:The detection and clinical significance of salivary enzymes,IL-18inpatients with Oral lichen planusObjective: This study designed to analysis whether ALT,AST,LDH areassociated with the occurrence of OLP by detecting the level of them in saliva ofOLP patients before and after treatment and healthy people.Analyse whether IL-18is associated with the occurrence and development of OLP by detecting theexpression of IL-18in OLP tissues and normal oral mucosa tissues.Methods: Automatic biochemistry analyzer was used to detect ALT, AST,LDH levels in saliva of30patients with OLP before and after treatment and30healthy people. Immunohistochemistry was used to detect IL-18in30cases of orallichen planus tissues and20cases of normal oral mucosa tissue expression,toanalyse the differential expression in these two tissues.Results:1Oral lichen planus patients before treatment, ALT, AST levels in salivacompaired with them in healthy control group and after treatment showed nosignificant difference(P＞0.05). LDH levels in saliva before treatment wassignificantly higher than the healthy control group, the difference has statisticalsignificance(P＜0.01),and decreased significantly after treatment(P＜0.01).2IL-18which mostly expressed in cytoplasm,was expressed in normal oralmucosa tissue and oral lichen planus.Its expression in oral lichen planus was muchstronger than that in normal mucosa tissues(Z=-4.314，P=0.000).Conclusion:1ALT, AST level in saliva have no obvious relationship with OLP;and higherLDH content in saliva, may be associated with the occurrence of OLP. 2IL-18expression level in oral lichen planus tissue was higher than thatin normal mucosa tissue,which had statistical difference. IL-18could inducethe regulation of cytokines, to destroy the Th1/Th2subsets balance and lead tothe occurrence of OLP.