| Background Nasal polyps (NP) is one of the most common disease in Otorhinolaryngology, often originate from paranasal sinus and middle nasal meatus.The clinical manifestation of NP is regional oedema and semitransparent apophysis of nasal mucosal. Surgical treatment is the most common therapy for NP. And it's recurrence rate after surgery is at a high level. Pathology character of NP includes hyperplasia of goblet cell, incrassation of basilar membrane, infiltration of white blood cell(especially acidophilic granulocyte). The pathogenesis of NP is still insufficiently understood. Recent research demonstrated that inflammation, infection and allergy play important roles in pathogenesis of NP. Existence of effector cell and cytokine related to inflammation, infection and allergy was also reported. The latest reserch demonstrated that origination and progress of NP was regulated by some specific genes. Limited by experimental technique, research inland is still operated by traditonal methods and did not demonstrated NP's pathogenesis at genomic level. As a new type of experimental technique, GeneChip has obtained lots of achievements in scientific research. It becomes one of the most effective methods in experimetal research.Objective Research alteration of gene expression profiling in NP using GeneChip, explore pathogenesis of NP.Methods HG-U133A2.0(Affymetrix Corporation) was used to test 5 simple NP samples,4 NP samples combined with asthma and 5 normal mucosal. Dianose of NP and asthma was according to results of pathological examination and pulmonary function test, respectively. Original data from GeneChip was standardized using RMA software, then statistical analysis was performed by SAM software. After screening out the significative target genes, drawing them using IPA software and analyse the typical differential expression genes and their changes of signal channel.Result The difference of gene expression in simple NP and NP combined with asthma did not reach statistical significance using SAM software.2122 differential expression genes (871 down-regulated genes and 1251 up-regulated genes) were screened out using SAM software (FDR=5.8). Fold Changes in 533 down-regulated genes and 486 up-regulated genes were greater than 0.5,2, respectively. Using IPA software, signal transducting system of TGF, arachidonic acid and complement was mostly found up-regulated in NP.Conclusion 1. TGFβwas obviously up regulated in NP, and expression of cytokine in its signal channel was obviously changed. It demonstrated that immunological factor, infiltration of acidophilic granulocyte, and deposition of matrix protein may play a important role in NP origination.2. Complement activation in NP was obviously increaced compared with normal nasal mucous, which proved that inflammatory factor and metabolite of inflammatory cells may play a important role in NP origination.3. Expression of leukotrienes and prostaglandin E2 was obviously changed in NP, which demonstrated that allergy may be an important aetiological agent in NP. |
