Derivation Of Neuronal-like Cells From Multipotent Human Follicular Stem Cells

The optimal source of stem cells for regenerative medicine is a major question. Embryonic stem (ES) cells have shown promise for pluripotency but have ethical issues and potential to form teratomas. Pluripotent stem cells have been produced from skin cells by either viral-, plasmid- or transposon-mediated gene transfer. These stem cells have been termed induced pluripotent stem cells or iPS cells. iPS cells may also have malignant potential and are inefficiently produced. Embryonic stem cells may not be suited for individualized therapy, since they can undergo immunologic rejection.Recent researches found that hair follicle contains a variety of stem cells e×cepts epidermal stem cells and melanoma cells . hair follicle pluripotent stem cells present in the hair follicle dermal papilla and dermal sheath with self-renewal ability and differentiation potential. under certain condition cells can differentiate into a variety of cells. The cells in the adult dermal papilla and dermal sheath are well-characterised particularly because of their remarkable inductive powers.Directly isolated and cultured dermal cells from mature adult follicles have been shown to be capable of inducing a range of epithelia to form follicles.multipotent human follicular stem cells in the body with slow periodic, but have high proliferative capacity specific in vitro conditions. multipotent human follicular stem cells have self-renewal ability and differentiation potential, under certain conditions cells can differentiate into a variety of functions. multipotent differentiation capacity is the most important characteristic to the identification of stem cells. More and more research have proved that mesenchymal stem cells can different into a variety of cell by given the cultivation of different cell differentiation conditions.Our e×periment is studying on the multipotent human follicular stem cells'segregation, culture and identification. In e×periment, we obtained the human hair follicle-derived mesenchymal-like stem cells simply by plucking the adults'hair . The flow cytometry analysis shows their high e×pression of mesenchymal stem cells related markers , including: CD29,CD44,CD73,CD90,CD105;rare e×pression of epidermal stem cells marker CK15, hematopoietic stem cells marker CD34,leukocyte marker CD45,endothelial cell marker CD31 and immune response-related major histocompatibility antigen HLA-DR.All the above indicates that these cells are rich in mesenchymal cells. The multipotent human follicular stem cells showed adipogenic and osteogenic capacity and can different into neuronal-like cells with db-cAMP and IBMX. So multipotent human follicular stem cells may treat spinal cord injury, Parkinson's disease and other nervous system disease one day.