The Study Of The Role Of Chemokine MCP-1, MIP-1α And MIP-1β In Children With Type 1 Diabetes Mellitus

ObjectiveChemokine is a complex of small molecular weight secreted protein superfamily. Recent studies show a wide range of inflammatory and autoimmune diseases has been observed upward or downward chemokines and chemokine receptors, can affect susceptibility, development and severity of disease.The aim of this study is to compare the serum levels of MCP-1, MIP-1αand MIP-1βin children with Type 1 diabetes mellitus and healthy children,and to explore the role of chemokine MCP-1, MIP-1αand MIP-1βin children with Type 1 diabetes mellitus.Methods51 Type 1 diabetes mellitus children were recruited as study group, including 30 children with Initial diagnosis of Type 1 diabetes mellitus,8 children with insulin treatment for 3 months,4 children with insulin treatment for 6months and 9 children with insulin treatment for 12 months.All the Type 1 diabetes mellitus children met diagnostic criteria recommended by 1997 American Diabetes Association (ADA). 29children as normol controls. Serum levels of chemokine MCP-1, MIP-1α, MIP-1βwere measured. At the same time fasting blood gulcose, C-peptide, glycosylated hemoglobin(HbAlC) in study cases were routinely send for detection. The significance and correlation among these parameters were analyzed.ResultsLevels of serum MCP-1, MIP-1αin study group were significantly higher than controls (P＜0.05, P<0.01). While levels of serum MIP-1βin study group had no significant difference compared with controls.Levels of serum MCP-1, MIP-1αfor newly diagnosed type 1 diabetes mellitus were significantly higher than controls (P＜0.05, P＜0.01). Levels of serum MCP-1, MIP-1αin children with 3 months insulin therapy were significantly higher than controls (P＜0.05, P＜0.05). Levels of serum MCP-1, MIP-1αin children with 6 months insulin therapy were significantly higher than controls (P<0.01, P<0.05).Levels of serum MCP-1, MIP-1αand MIP-1βin children with 12 months insulin therapy were not significantly different with controls.MCP-1 in newly diagnosed group,3,6 and 12 months insulin therapy between the two groups was statistically significant (P＜0.05), but MIP-1α,MIP-1β(P>0.05, P>0.05) in the above groups were not significatly different.There is no correlation between Levels of serum chemokine in study group and various biochemical parameters (P> 0.05).ConclusionThis study demonstrated that children with type 1 diabetes onset in vivo existence of Th1/Th2 immune imbalance. chemokine MCP-1, MIP-1αis involved in the process and progress as the condition persists, suggesting that chemokine MCP-1, MIP-1αmay play an important role in the pathogenesis of type 1 diabetes in children.