| BackgroundCondyloma acuminatum(CA) is a benign papillomatous hyperplasia caused by infecting human papilloma virus(HPV) to human genitals and the surrounding skin. The typical biopsy change is the vacuolated cell presenting in the upper epidermis. The therapies of the disease are not difficult, but the high recurrence rate and increasing incidence rate are attracting people's attention gradually. It widely recognized that change of organism immune function closely relates to the occurrence and turnover of CA. The suppression of patients'immune system especially cellular immune function is the main reason for HPV persistent infection.Toll-like receptors (TLRs) are a kind of recently identified patterns recognition receptors. they widely distribute in the surface of cells and join the identification of pathogenic microorganism and their products. And they can clear the pathogenic microorganism and regulate immune status through inducing the production of reactive oxygen intermediates and reactive nitrogen intermediates and the release of proinflammatory factors. They play important roles in inherent immunity defense, and have always been considered as the joint between inherent immunity and adaptive immunity which can impact adaptive immunity further. The function of TLR has been paid more and more attention in CA. In the 11 TLR family members that have been found, TLR3 and TLR9 are considered as two important receptors in anti-virus immune response. In this research, we attempts to reflect the immune state of CA patients' organism from a new perspective so that we can find a better therapeutic way to clear HPV and improve CA patients'prognosis through observing the expression of TLR3 and TLR9 in CA patients'skin lesions with different prognosis.ObjectiveTo observe the expression of TLR3 and TLR9 so that to further explore their roles in pathogenesis of CA and the relationship with the occurrence and the development of CA.MethodsCollect 24 CA patients (14 cases in non-recurrence,10 cases in recurrence) and 10 healthy controls. The expression of TLR3 and TLR9 in each group of CA and normal prepuce skins was detected by immunohistochemistry staining and RT-PCR method. All the datas are expressed by mean±standard. The software SPSS13.0 was applied for statistical analysis. T test analysis was used to compare cases and controls. The test results have statistical significance when P<0.05.Results1.Location analysis by immunohistochemistry staining:TLR3 and TLR9 proteins localized in the cytoplasm and (or) cell membrane of keratinocyte. In normal controls, the expression of TLR3 and TLR9 was mid-strong or weak positive throughout the epidermis; In non-recurrent group, the expression of TLR3 and TLR9 was strong positive throughout the epidermis, and mainly located in spinous layer and granular layer; In recurrent group, the expression of TLR3 and TLR9 was mid-strong positive throughout the epidermis, and mainly located in basal layer and spinous layer.2.Semiquantitative analysis by RT-PCR:The expression of TLR3 and TLR9 mRNA in non-recurrent group was higher than that of normal controls(P<0.01; P< 0.05), and the expression of TLR3 mRNA was much higher than TLR9 mRNA (P< 0.05); The expression of TLR3 and TLR9 mRNA in recurrent group was similar with that of normal controls (P>0.05), but the expression of TLR3 mRNA in recurrent group was much lower than that of non-recurrent group (P<0.05).Conclusion1. The expression of TLR3 and TLR9 was up regulated in non-recurrent CA;2. The expression of TLR3 and TLR9 in recurrent group was similar with that of normal controls (P> 0.05). Their expressions were reduced in different degree compared with non-recurrent group, and the decrease of TLR3 in recurrent group was significant (P<0.05);3. There were differences between the recurrence CA and non-recurrent CA for TLR3 and TLR9's expression positions;4. The changes of TLR3 and TLR9's expression position and level may be associated with the prognosis of CA, and TLR3 may exert greater impact on it. |
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