Expression Of Toll Like Receptors 7 And 9 In Lesions Of Primary Condyloma Acuminatum

Objective: In this study,we observed the distribution and expression of Toll-like receptors 7 and 9 in the lesions of initial patients with condyloma acuminatum(CA),and explored the possible role of Toll-like receptors 7 and 9 in the pathogenesis of CA and their relationship.The results provide theoretical basis for the prevention and treatment of condyloma acuminata and other related diseases,and accumulate some experimental data for further study.Methods: Immunohistochemistry was used to detect the distribution and expression of Toll-like receptor 7 and Toll-like receptor 9 in the skin lesions of the untreated patients with condyloma acuminate(experimental group)and the normal prepuce(control group).Statistical analysis using the social sciences statistical software package(SPSS 18.0),The data between the experimental group and the control group were compared with chi-square test,P < 0.05 considered as statistically significant difference between the two.Results:1.TLR7,TLR9 distribution and expression: TLR7 showed moderately positive to strongly positive staining in stratum spinosum,stratum granulosum and stratum corneum in the lesions of patients with condyloma acuminatum(experimental group),and weakly positive to moderately positive in stratum basale.In the foreskin(control group),TLR7 was weakly positive in stratum spinosum and stratum granulosum,and negative to weakly positive in stratum basale and the stratum corneum.The expression of TLR9 in stratum basale,stratum corneum,stratum spinosum and stratum granulosum showed a moderate positive intensity to a strong positive expression,especially in stratum spinosum,and even in part of the cytoplasm the accumulation of particulate matter can be seen(strongly positive performance).While in the control group,TLR9 showed weakly positive expression in stratum spinosum and stratum granulosum,but no significant positive expression in the remaining epidermal cell layer.2.Image processing and statistical analysis of the results: The expression of TLR7 and TLR9 in lesions of patients with condyloma acuminatum(experimental group)mostly showed moderately to strongly positive expression.Statistical analysis showed that the positive rate of expression of the two in the experimental group and the control group was statistically significant.(P <0.05)3.The correlation coefficient of TLR7 and TLR9 expression intensity in patients with condyloma acuminatum was r=0.114,P =0.548(P> 0.05).There was no correlation between TLR7 and TLR9 in the onset of condyloma acuminatum.Conclusion:1.The positive expression rate and expression intensity of TLR7 and TLR9 in lesions of patients with initially condyloma acuminatum were significantly higher than those in normal foreskin tissues,suggesting that both TLR7 and TLR9 were involved in the pathogenesis of condyloma acuminatum.2.There is no correlation between the expression levels of TLR7 and TLR9 in lesions of patients with initially condyloma acuminatum.It may be necessary to increase the sample size and replace experimental methods that can be quantitatively analyzed.3.The positive expression rate and expression intensity of TLR7 and TLR9 in the lesions of patients with condyloma acuminatum were higher,suggesting that it may provide a new theoretical basis for the treatment of condyloma acuminatum.