Electrochemical In-Situ Investigation On The In-Vitro Biomaterials-Related Thrombosis

The mechanism of inorganic biomaterials-related thrombosis has not yet been fully understood. Fibrinogen is considered a key coagulation factor, playing an critical role in the biomaterials-related thrombosis. According to the electrochemical point view regarding the inorganic materials-related thrombosis, the cascade process depends on the electrochemical charge transfer and physical chemistry reactions between the interface of the fibrinogen and materials. The current studies mostly focus on fibrinogen adsorption of biological materials and the deformation behavior. Thus, studies on the interfacial physiochemical reactions between fibrinogen and materials are of great significance.In this work established a set-up of in situ electrochemical system to investigate the inorganic materials related coagulation process, through combination of in situ electrochemical methods and biological evaluation to study the electrochemical parameters of fibrinogen denaturation and platelet adhesion. Furthermore, we have explored the relation between fibrinogen activation and platelet adhesion behavior.1) By changing applied potential on the surface of the material (316L stainless steel and graphite) in PBS and PBS+ Fbg solution system, we obtained interfacial current density and current versus time curve under different voltages.2) We carried out also two ELISA methods on electrochemical pre-adsorbed fibrinogen sample surfaces to detect the denaturation of fibrinogen: quantitative testing of exposed fibrinogenγchain and quantification of release of FPA peptide.3) Platelet adhesion was carried out on pre-adsorbed fibrinogen surfaces, then followed by LDH test to quantify the amount of adsorbed platelet.4) In situ electrochemical adsorption of PRP, adsorption of platelets was quantified by LDH test. Based on the biological evaluation results and electrochemical test results, we correlated fibrinogen denatraiton with platelet adhesion under different applied potentials. Finally, we summarized and analyzed the influence of the electrode potential on behavior of fibrinogen and platelets, and further explained the electrochemical reaction between fibrinogen and the materials from point view of the electrochemical mechanism of thrombosis.According to the electrochemical results, on the surface of 316LSS and applied with anodic potential, the current density of PBS+Fbg