The Effect Of Insulin-like Growth Factor 1 (IGF-1) And Exercise On The Protein Expression Of Mammalian Target Of Rapamycin (mTOR) And Androgen

The study examined the effects of IGF-1 injection and exercise on the protein expression of mTOR, AR in rats.24 male Sprague-Dawley rats were divided into four groups:inactive control(C), inactive plus IGF-1 injection (CI), exercise (E), exercise plus IGF-1 injection (EI). After all groups had been accustomed for one week, the Group E & EI performed closely monitored training in the treadmill (grade of 5, at the speed of 20m/min, duration of 1 hour) for 7 days. When the exercise programs were completed every day, IGF-1 was injected subcutaneously in the back of right leg immediately. After 6 hours of the last injection, tibialis anterior muscle (TA) was isolated and IGF-1 level, the protein expression of mTOR and AR were determined. In our study, exercise evoked a significant (p<0.05) increase in IGF-1 level and mTOR protein expression in the TA. Compared with Group C, there was a downtrend in AR protein expression in other three groups, without significant difference (p> 0.05). The results indicate that one week endurance exercise stimulus induces an increase in IGF-1 level and mTOR protein expression. But the phenomenon of AR decrease should be taken further verification.