| Cerebral infarction disease belongs to the concept of "Zhongfeng" in traditional chinease medicine. Many data indicates that, Chinese acupuncture have a novel effect on restoration of limb function after cerebral infarction and Cerebral Hemorrhage. According to traditional meridians and collaterals theory, "baihui" (Du Mai 20) is the acupoint where the body's Yang energy naturally converges. Also, it can regulate cerebral and vascular function, improve the intelligence. "Zusanli" (Stomach-36) is the He-Sea of stomach channel of foot yangming, which is rich in qi and blood and related to Spleen Meridian of Foot-Taiyin.Research objective:According to meridians and collaterals theory of traditional chinese medicine, our experiment choose the high frequency acupoints "Zusanli" and "Baihui " and the middle cerebral artery occlusion model on rats to explore its effect on the cerebral expression of inflammatory related molecular ICAM-1 and extracellular matrix destroy related molecular MMP-9 after cerebral ischemia-reperfusion injury in rats.Research methods:male SD rats was induced MCAO model,the rats was randomly divided into seven groups:sham group, model group,Electro-acupuncture at "Zusanli" and "Baihui " group, "Baihui " group, Nimodipime group, joint group with acupuncture and Nimodipime (EA+N group for short). Electro-acupuncture group, model group and sham group have six small groups according to different time points at 12,24,48,72,96 and 144 h; "Baihui " group, Nimodipime group, EA+N group each was divided into 12,24,48,72h 4 time points; besides, control group have 7 rats. We explored the expression of MMP-9 and ICAM-1 in rats brain tissue by immunohistochemistry staining; And the peripheral blood sample was analyzed by Surface Enhanced Laser Desorption Ionization-Time Of Flight-Mass Spectrometry technique to see the difference of protein expression pre-and post acupuncture therapy.Research results:1. TTC staining show that the core infarction area is white in colour, compared with the common red brain tissue. Among the five slices of brain, the slices around optic chiasma area had the most stable infarction region.2. HE staining show the pathological lesion is consistent with the TTC staining results. The lesion reached the peak at 48th hour in the infarct core area; until the 72th hour, the cerebral interstitial oedema was alleviated. Also many glial cells and collagen fibers proliferated. Up to 144h, the proliferation of glial cells and collagen fibers was the most obvious.3. Immunohistochemistry staining:1) the expression of MMP-9 and ICAM-1 exhibited a double-peak profile at the 48th and the 144th hours; 2) electro-acupuncture group can down-regulate the expression of MMP-9 and ICAM-1 in brain tissue at the time point of 48h, but the lever was still above the the level of sham goup and the control group; while at the time point of 144h, the therapy had the opposing effect; 3) at the functional recovery stage,the newborn glial cells and neurons which presented the signal of restoration proliferated. In model group the signal appears at 96h, while in electro-acupuncture group was 24h earier.4. SELDI analsis indicates that there are 107 significant different protein peak and trough between electro-acupuncture and model group (50 peak and 57 trough). Among all these 107,11 were in 12h group; 22 in 24h group; 7 in 48h group; 42 in 72h group; 22 in 96 group and 3 in 144h group.Research conclusions: Acupuncture at the acupoints of'Zusanli" and "Baihui " may have good effect on the inflammatory related injury after brain infarction by upregulating and down-regulating the expression of MMP-9,ICAM-1,the proliferation of glial cells and neurons. It showed that acupuncture had the dual regulated effect.
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