| Objective:In this experiment study, the artificial construct five dogs with chronic periodontal disease model, and then the recombinant human basic fibroblast growth factor (recombinant human basic fibroblast growth factor, rhbFGF) and recombinant human insulin-like growth factor (recombinant human insulin-like growth factor-I, rhIGF-I), either separately or simultaneously with the artificial bone (Bio-oss) composite, implanted into dogs degreeâ…¡furcation periodontal disease model, and with guided tissue regeneration (Guided issue regeneration, GTR) treatment, observation of animal models of periodontal tissue repair and regeneration, and to explore two kinds of growth factors to promote periodontal tissue regeneration and the effect of clinical treatment of periodontal disease in itsapplication to provide a theoretical basis.Methods:1. The formation of five dogs with chronicâ…¡furcation of the mandibular first premolar 2,3,4, for a total of 48 teeth were randomly divided into 5 groups, including the three experimental groups, a control group and a blank control group, experimental group A:rhbFGF/rhIGF-I/ Bio-oss/GTR; experimental group B:rhbFGF/Bio-oss/GTR; experimental group C:rhIGF-I/Bio-oss/GTR; the control group D:GTR surgery alone; the blank control group E:flap operation.2. Before implant rhbFGF and/or rhIGF-I composite bone graft and after 12 weeks,record buccal side of the bifurcation site of periodontal probing depth (periodontal depth,PD)and attachment level (attachment level,AL);after 2,4,12 weeks taken surgery X-rays to observe the new situation of alveolar bone.3. Dogs were sacrificed 12 weeks after surgeried, slice the preparation experimental tissue zone (including dental, alveolar bone, periodontal ligament), HE, Masson staining and histological measurements, observe periodontal tissue (alveolar bone, teeth bone and periodontal membrane) of the new situation.Results:1. The clinical examination:after 12 weeks the PD and AL of dogs's model were improved to varying degrees, followed by A, B/C, D and E group;2. X-ray examination:the experimental group A, B, C alveolar bone almost are filling the molar furcation, and the bone can be seen more clearly hard board; control group D were higher than the experimental group, less new bone, but more than the control group E; control group E are filling with a few sparse trabecular bone in the bottom of the low bone mineral density, bone hard plate fuzzy;3. histological observation:HE staining, the experimental group A, B, C shows that new bone defect filling furcation, which become higher bone mineral density, calcium good; new born periodontal fiber small dense, rich in blood vessels, the direction perpendicular or nearly perpendicular to the root surface; new cementum covering most areas of the root bifurcation; the experimental group showed no bone adhesion and root absorption of malunion. Control group D, some new alveolar bone was layered over mature cementum covering the root surface to the coronal extension; fibers within the periodontal gap crossed. The control group E appears only a small number of individual defect new bone, and it has more epithelial cells and non-bone connective tissue covering. Masson staining in the experimental group A, B, C showed obvious red stained area with peripheral small amount of blue stained area; control group D Masson staining red-blue, red area of trabecular bone center; blank control group E showed obvious blue, surrounded by a small amount of red staining area;4. tissue measurements can be seen in all experimental groups a high degree of new bone, new cementum height and new indicators of periodontal tissue were higher than that and the control group (P<0.05), compared with the experimental group, A group of the indicator, although numerically higher than B, C group was slightly higher, but the statistical analysis, three groups were not significantly different (P>0.05).Conclusion:1. Through artificial canine furcation bone defect site and supported by a stable method of local incentives that can be prepared reliable and similar to clinical periodontal disease in dogs II furcation Animal models, to be used for a variety of periodontal tissue regeneration therapy in animal study.2. By clinical examination of animal models, histological observation, histological measurements, the results show rhbFGF and/or rhIGF-I and Bio-oss composite material has good bone and bone induction guide for dog II degree of root bifurcation defects can significantly promote alveolar bone, cementum and periodontal regeneration and new attachment formation.3. The experimental results proved to use rhbFGF and/or rhIGF-I and bone composite to treatment canine periodontal diseaseâ…¡furcation periodontal regeneration model is superior to simple guiding periodontal tissue regeneration.4. The experimental results show that alone rhbFGF, rhIGF-I and these two factors combination on the periodontal tissue regeneration (regeneration of alveolar bone, cementum regeneration, periodontal membrane formation) the quality was no significant difference.
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