The Experimental And Clinical Research Of Spinal Cord Injury Treated By Hyperbaric Oxygen

Part 1: Effects of Hyperbaric oxygen thrapy on inflammatory factors after spinal cord injury in ratsObjective To investigate the changes of inflammatory cytokines in serum of rats and motor functional recovery after spinal cord injury treated by Hyperbaric Oxygen Therapy and potential therapeutical mechanisms.Methods The spinal cord injury models were established with SD rats according to Allen,s method .75 SD rats were randomly divided into three groups:sham operation group(A,n=25), control group(B,n=25), hyperbaric oxygen group(C,n=25). After the spinal cord injury models were established ,the rats of C group were treated by Hyperbaric Oxygen,100 minutes per every time,once per day for totally five days. After treatment,the neurological outcomes of rats were evaluated at 4 hours,12 hours,1days,3days,and 5days with BBB scores ,at which time the levels of inflammatory cytokines,such as TNF-α,IL-6,IL-8 and IL-10 in serum of rats were measured by radioimmunoassay(RIA).Results Compared to the sham operation group, the levels of inflammatory cytokines of HBO group and control group were increased at different time points after spine cord injury (P<0.05).These parameters were decreased obviously in the HBO group with the comparison of those in the control group ( P < 0. 01).The BBB scores of HBO group was higher than control group, s(P<0.05).Conclusion HBO could protect the injured myeloid tissues and lessen secondary spinal cord injury by inhibiting the infiltration of inflammatory cells and decreasing the levels of inflammatory cytokines after SCI. Part 2:Effects of Hyperbaric oxygen on erythropoietin and its receptor after spinal cord injury in ratsObjective To observe the changes of the expressions of erythropoietin(EPO) and its receptor(EPO—R)in the spinal cord injury after Hyperbaric oxygen therapy potential mechanisms.Methods The spinal cord injury models were established with SD rats according to Allen,s method.75 SD rats were randomly divided into three groups : sham operation group(A,n=25), control group(B,n=25), hyperbaric oxygen group(C,n=25). After the spinal cord injury models were established ,the rats of C group were treated by Hyperbaric Oxygen,100 minutes per every time,once per day for totally five days. The samples were taken from the spinal cord injury sites at 12h, 24h, 2d, 7d and 14d after Hyperbaric Oxygen Therapy. The levels of EPO and its receptor were detected by RT-PCR and ELLSA.Results The expressions of erythropoietin receptor (EPO-R) of experimental group were higher than the other two groups at each time point(p<0.05).The levels of EPO did not significantly change.Conclusion The higher expressions of erythropoietin receptor (EPO-R)in the spinal cord was one of the protective mechanism of Hyperbaric oxygen after acute traumatic spinal injury.Part 3:Clinical effects of hyperbaric oxygen therapy on the patients with actual spinal cord injuryObjective To observe motor functional recovery of the patients with acute spinal cord injury treated by hyperbaric oxygen.Methods 47 cases of patients with acute incomplete spinal cord injury, after 5 days - 10 days of hyperbaric oxygen therapy started, the treatment of the three treatment; once a day for each 90-min treatment time, 10 days for a course of treatment, Interval two days between two courses of treatment.Hyperbaric oxygen therapy in the course of treatment before, ASIA score is conduted.Results After three courses of hyperbaric oxygen treatment, 66% of patients improve ASIA score on the hyperbaric oxygen therapy before and after there was a marked difference..Conclusion HBO can significantly promote the recovery of SCI patients.