| Objective: To study the mechanism of different doses of artemisinin on human gastric cancer cell line SGC-7901 implanted under the skin of nude mice in vivo.Methods:Human gastric cancer cell line SGC-7901 was transplanted to subcutane of nude mice to establish the sample and then randomly divided into five experimental groups, which include artemisinin-low-dose group (100mg/kg), artemis-inin-medium-dose group (150mg/kg), artemisinin-high-dose group (200mg/kg), blank control saline group and the positive control 5-FU group (20mg/kg), each of which was compose of five nude mice and then Continuously received gavage for 10 days in nude mice, sacrifice the nude mice within 24 hours after the withdrawal, weighting the transplanted tumor, calculate the anti-tumor rate, The apoptotic rate by flow cytometry and the activity of Caspase-3 by Microplate Reader.Results:The inhibition rates of high, medium and low-dose artemisinin-based group and 5-FU group were 71.4%,47.7%,31.3% and 45.2% Separately, compared with the saline group werestatistically significant (P<0.05), low-dose group compared with 5-Fu group, the inhibition rate P= 0.0457<0.05, that is statistically significant; However, can be seen from the inhibition rate effects of 5-Fu group better than the artemisinin-low-dose group .High, medium and low-dose artemisinin-based group and 5-Fu group by flow cytometry detection of apoptosis were apparent distribution, with apoptotic rates were78.0ï¼…,65.9ï¼…,45.9ï¼…,57.9ï¼…, Caspase-3 activity detection by microplate reader was found to increase with the dose of artemisinin, which increased activity of high-dose artemisinin-the most significant.Conclusion:Artemisinin can inhibit human gastric cancer cell line SGC-7901 in nude mice subcutaneously transplanted tumor growth, through the activation of Caspase-3 induced cell apoptosis as an anti-tumor effect in vivo.
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