The Effects Of Somatostatin On The Proliferation In Human HepG2 Cells And The Level Of Gene P16

Objective Through using the Flow cytometry and Reverse transcription to determine the Human HepG2 Cells which is treated by somatostatin, we study of somatostatin and its analogues on the human HepG2 Cells growth mechanism and the P16 gene expression levels in order to investigate the inhibition of hepatoma cells or apoptosis mechanism.Methods The cell line HepG2 were divided into 3 groups:control groupA, low concentration groupB and high concentration group C. The flow cytometry (FCM) was used to detect phase distribution o fthe cycles and detect the expression of p16 gene by using RT-PCR.。Results Human HepG2 Cells affected 24 hours later by different density SST, using FCM,we can discovered that the G1 cell cycles of each A group,B group,C group occupied the proportion respectively is 51.41±3.27%.65.426±3.41%,69.019±3.75%, that the S cell cycles is 36.86±2.31,20.343±2.52%, 18.502±3.09%, that the G2 cell cycles is 11.46±1.96%,14.231±3.83％,12.479±3.18%。Compare to the control groupA,the G1 cell cycles of groupB and group C were increased obviously.But the S cell cycles relative reduction.The levels of P16 gene were expressed in each group detected by RT-PCR,but B group and C group expressed obviously.Conclusions1.Somatostatin and its analogues inhibit the malignant human hepatoma cell proliferation.2.Somatostatin and its analogues inhibit the malignant proliferation of HepG2 hepatoma cells concerned with the concentration.3.Somatostatin and its analogues inhibit the proliferation of human hepatoma cell concerned with P16 expression.