Mechanisms Of Pab Blocking HepG2 Cell Cycle And Inhibiting Its Invasion And Migration

ObjectiveTo study the mechanisms of PAB blocking Hep G2 cell cycle then inhibiting the proliferation and inhibiting the invasion and migration of Hep G2 cells.MethodsThe effect of PAB on the proliferation of Hep G2 cells was observed by MTT assay;The morphological changes of Hep G2 cells were observed by optical microscope;The effect of PAB on the cell cycle of Hep G2 cells was detected by flow cytometry;immunofluorescence,fluorescence the impact of PAB on the expression and aggregation morphology of ?-tubulin;Wound healing assay and Transwell chamber invasion assay were used to detect the effect of PAB on the migration and invasion ability of Hep G2 cells.The effects of PAB on the expression of ?-tubulin,E-cadherin and MMP-9 were detected by Western blot.Results1.After treated with different concentrations of PAB,the survival rate of Hep G2 cells decreased gradually and compared with the control group,the difference was statistically significant(P<0.05).The IC50 was 2.23 ?mol/L;2.After treated with different concentrations of PAB,the number of cells in G2/M phase gradually increased and compared with the control group,the difference was statistically significant(P<0.05);3.After treated with PAB at different concentrations,Hep G2 cells were observed by fluorescence microscopy.The results showed that PAB changed the morphology of tubulin and decreased its expression significantly;4.After treated with PAB at different concentrations,scratch healing results showed that the cell migration distance in PAB treated group was significantly shorter than that in control group(P<0.05).Transwell chamber invasion assay showed that the number of transmembrane cells in PAB treated group decreased than that in control group(P<0.05).5.Western blot results showed that the expression of ?-tubulin decreased,the expression of E-cadherin gradually increased and the expression of MMP-9 gradually decreased after treated with PAB at different concentrations.Compared with the control group,the differences were statistically significant(P<0.05).Conclusions1.PAB can inhibit the proliferation of Hep G2 cells by affecting the aggregation morphology and down-regulating the expression of ?-tubulin,arresting the cell cycle of Hep G2 cells in G2/M phase.2.PAB can inhibit the invasion and metastasis of Hep G2 cells by down-regulate the expression of ?-tubulin and MMP-9,up-regulate the expression of E-cadherin.