The Experiment To Improve Effects Of Vitamin A Deficiency And Preliminary Study Of Vitamin A Product

Vitamin A deficiency (VAD) is one of the most common micronutrient deficiencies in children under the age of 6 years in the world. As known to all, deficiency of vitamin A will lead to dysfunction of the immune system, growth retardation. Studies also suggest VAD, sub-clinical VAD (SVAD) and even the state of slightly low vitamin A may increase childhood morbidity and mortality. Therefore, it is extremely important to study the effect of VAD intervention and seek the suitable intervention way. Moreover, there will be important public health implications for regions of the world where vitamin A deficiency is prevalent. The aim of this research was to get the best vitamin A combination through studying the effect of supplement combination of vitamin A,zinc and iron on vitamin A deficiency rat. On the basis of previous study, we manufactured a primary vitamin A product. It is hoped that this product will increase the vitamin A level of children who lack of vitamin A and will promote growth and development of children. Objective: The objective was to find out the best vitamin A combination through studying the effect of supplement combination of vitamin A, zinc and iron on vitamin A deficiency rat.Methods: SD rats (Sprague Dawley rats) were used as animal model based on the reforming formula of American Institute of Nutrition (AIN-93). After vitamin A deficiency model was done successfully, the nutrition intervention experiment was carried out on these rats, which were randomly divided into eight supplement groups intervened for eight weeks (VA, VA+Zn;VA+Fe;VA+Zn+Fe;1/3VA;1/3VA+Zn;1/3VA+Fe and 1/3VA+Zn+Fe supplement group). Vitamin A content in serum and liver, zinc and iron content in serum, the blood convention and biochemical indicator were detected before intervention, 4 weeks after intervention and at the end of intervention, respectively. The weight and food ingestion were monitoring at the same time.Results: The study showed that vitamin A content in serum and liver, the weight and food ingestion were significantly increased in all supplement groups after the intervention. Compared to the control group, the supplement groups showed no significant differences in blood convention, biochemical indicator and zinc and iron content in serum. Vitamin A content in the 1/3VA+Zn group was significantly higher than other 1/3-groups. But it had no significant difference compared with the whole-groups or the normal control group, same as the increased weight, the whole food ingestion and efficiency of food ingestion.Conclusion: Supplements can obviously increase the nourished status of vitamin A and the group of 1/3VA+Zn was best vitamin A combination. That will provide a reliable base for further study of vitamin A product. Objective: The purpose of this study was to produce preliminarily nutrition flour to improve vitamin A status of children who suffer from vitamin A deficiency on the basis of rat experiment.Methods: According to efficiency ofβ-carotene convert to vitamin A in vivo, carrot flour which is rich inβ-carotene and wheat embryo flour which is rich in zinc were mixed scientifically to make the product.β-carotene and zinc were detected repeatedly. The compounding was adjusted according to the content ofβ-carotene and zinc. Finally, contents of fat, ash, water copper, plumbum and arsenicum were detected respectively.Results: The vitamin A product was successfully finished. The content ofβ-carotene, zinc and fat was 145.4μg /100g,7.7mg/100g, 7.9%. All physical and chemical indexes meet the requirements of solid beverage. The product was analyzed by organoleptic evaluation, and it reached children's demand.Conclusion: The content of zinc of this product reached recommended nutrient intake (RNI) of children aged 1-7years,β-carotene (transformed to vitamin A) reached the dose of 1/3RNI of 1-7year old children, and the taste was excellent. It will provide advantageous assistance for children's vitamin A deficiency condition.