Dose And Time Effect Of Clot Lysis With Urokinase In Vitro

ObjectiveTo explore the optimal dose and time for clot lysis with urokinase to guide the clinical application.MethodsAccording to the 5×5Latin Square design, analogueing minimally- invasive surgery, experimental tubes received 0.2ml of UK solution (1000IU,2000IU,4000IU,10000IU or 20000IU), injected into the central of the blood clot after blood samples for lh,2h,3h,4h or 6h. Then, volume of the residual clots were measured at 4h,6h,8h,16h or 24h after the UK injection. Application made in vitro model of cerebral hemorrhage (10% of the clinical reference design).ResultsSignificant difference (F= 3.917,P<0.05)of lytic efficacy of UKwas noted with UKdifferent dose treatment series,and lyticvolume (230.99mg)was significantly decreased with 20000IU/ml UK treatment compared with other two series(311.39mg, 302.29mg) between which no significant difference was found. There was no significant difference in lytic volume between therapy timing series(F= 0.75,P >0.05)or lysing time series (F= 1.26,P>0.05).ConclusionsThe results suggest that optimal dose of UK required to lyse 5 ml of clottedblood would be 4000IU,and delaying therapy of up to 24 hours does not significantly compromise its efficacy and 1 hour is quite sufficient for receiving good lytic efficacy with UKtreatment.