The Detection Of LN And UPAR In The Eutopic Endometrium Of Patients With Endometriosis And Its Significance

Backgrounds and ObjectivesAlthough Endometriosis is multiple common benign lesions, it has similar biological characteristics of malignant tumors. At present, preoperative diagnosis rate of endometriosis was low, Laparoscopy is the gold standard of the EMs diagnostic, but there is some shortcoming, such as traumatic, cumbersome and expensive procedure, etc, the clinical treatment of EMs is not yet unified, and there are several drawbacks. Providing appropriate clinical screening and intervention programs for EMs is necessary.The first step in the formation of the ectopic lesions of endometriosis is the endometrial cells separated from the original tissue, Laminin (LN) belong to adhesion molecules integrin protein family, the main components of the extracellular matrix (ECM), and important medium of the cell adhesion in the matrixin, after LN and its receptor combined. The key steps in the formation of the ectopic lesions of endometriosis is the degradation of extracellular matrix, urokinase type plasminogen activator receptor (uPAR) is an important member of extracellular plasminogen system, It can mediate the plasminogen activation, ECM degradation and invasive growth of cells in tissue components. Related different levels of LN and uPAR in endometrium of different individuals (endometriosis and non-endometriosis) and the clinical value this difference may produce, currently, there are few reported in the literature. In this study, some of the issues were preliminary studied.Materials and Methods1 Experimental groupsCollected endometrial tissue samples come from 84 cases of surgical treatment hospitalized patients in Second Affiliated Hospital of Zhengzhou University at February 2008 to February 2009,28 cases of pelvic EMs (12 cases of stageⅠ～Ⅱendometriosis,16 cases of stageⅢ～Ⅳendometriosis),20 patients of Adenomyosis group and 36 cases of non-EMs.2 Experimental methodsTo detect the OD values of LN/uPAR in endometrial tissue samples by Enzyme linked immunosorbent assay, followly, determine the protein concentration of samples by Bradford method, to obtain the LN/uPAR protein content in sample.3 Statistical analysis methodsApplicating SPSS 13.0 software conducts statistical analysis. All data are expressed as the form that means±standard deviation. Using single factor analysis of variance test conduct compare among the groups. Using group data t test conduct compare in internal group of Ems. Using Pearson rank correlation conduct analysis of correlation test, and all statistical methods with a= 0.05 as the level of significance.Results1 Compare the LN level in endometrial homogenate of three sets of data.The LN levels in tissue homogenate among the uterus endometrium of non-EMs, eutopic endometrium of patients with adenomyosis and Pelvic EMs were 61.02±7.45μg/mg Protein,53.03±7.22μg/mg Protein and 40.95±9.10μg/mg Protein, respectively. Compare the three groups, overall, the differences were statistically significant (F=24.895, P=0.001<0.05). Comparison was carried out between every two sets of data, the differences were significant all (P<0.05). The LN levels between stageⅠ～ⅡEMs and stageⅢ～ⅣEMs were 47.82±6.93μg/mg Protein and 35.61±6.68μg/mg Protein, respectively. Compare the two groups, the differences were significant (T=5.034, P=0.001<0.05).2 Compare the uPAR level in endometrial homogenate of three sets of data.The uPAR levels in tissue homogenate among the uterus endometrium of non-EMs, eutopic endometrium of patients with adenomyosis and Pelvic EMs were 3.77±0.71 ng/mg Protein,4.30±0.47 ng/mg Protein and 5.28±0.62 ng/mg Protein, respectively. Compare the three groups, overall, the differences were statistically significant (F=30.283, P=0.001<0.05), and and its level of uPAR gradually increased, the uPAR levels were of the highest in III-IV Ems. Comparison was carried out between every two sets of data, the differences were significant (P<0.05). Compare the LN levels between stageⅠ～ⅡEMs and stageⅢ～ⅣEMs were 5.06±0.55 ng/mg Protein and 5.45±0.62 ng/mg Protein, respectively. Compare the two groups, the differences were significant (T=-2.312, P=0.025<0.05).3 Analysis the correlation between LN and uPAR in adenomyosisThe LN and uPAR levels in eutopic endometrial homogenate were not correlated in Patients with adenomyosis (rp=0.154, P=0.518>.05).4 Analysis the correlation between LN and uPAR in Pelvic EMsThe LN and uPAR levels in eutopic endometrial homogenate were not correlated in Patients with Pelvic EMs (rp=0.079, P=0.669>0.05).Conclusions1 The LN levels in tissue homogenate among the Patients with uterus endometrium of non-EMs, eutopic endometrium of patients with adenomyosis and Pelvic EMs were differences, and the more severe EMs condition is, the lowest the LN levels is.2 The uPAR levels in tissue homogenate among the Patients with uterus endometrium of non-EMs, eutopic endometrium of patients with adenomyosis and Pelvic EMs were differences, and the more severe EMs condition is, the highest the uPAR levels is.3 Between the level of LN and uPAR in eutopic endometrium of patients with adenomyosis and Pelvic EMs were not relevant.