The Influence Of Zhi-Bai-Di-Huang-Decotion On UU-infected Rat's Spermatogenic Cell Apoptosis And Effect Factors Bax,Bak Of Mitochondria Expressions

Object:To observe the Influence of Zhi-Bai-Di-Huarig Decotion on spermatogenic cell apoptosis and the effect of apoptogenic Bax/Bak expression of Ureaplasma urealyticum(UU)-infected rats.Method:45 out of 60 male SD rats were randomly drawed and made into the UU infected animal models,the rest of which were the sham operation group.The UU infected animal models were then divided randomly into the model group,the minocyclin group and Zhi-Bai-Di-Huang Decotion group,each with 15 rats and dosaged with lavage.The model group and the sham operation group were lavaged with isotonic Na chloride,,while the minocycline group and Zhi-Bai-Di-Huang Decotion group were lavaged with the corresponding medicine.The rats were all killed after 21 days,the testicle weight,the UU cultivation results,the apoptosis rate of reproductive cells,the Bax and Bak expressive level of each groups were measured and compared.Result:The testicle weight,the positive rate of the UU cultivation results,the apoptosis rate of reproductive cells,Bax and Bak expressive levels and the classifications of the pathologic manifestations of the testicles between the model group,the sham operation group,the minocycline group and the Zhi-Bai-Di-Huang Decotion group, the differences were statistical significance(P<0.05).But the difference of the aforesaid index between the minocycline group and the Zhi-Bai-Di-Huang Decotion group didn't have any statistical significance.Conclusion:1.Zhi-Bai-Di-Huang Decotion can actively depress the growth and production of UU and is anti-UU.2.UU infection can lead to the increasing of spermatogenic cell's apoptosis in rats.It is proved that the over-apoptosis of spermatogenic cell is the important mechanism of the descending quality of the sperms after UU infection which leads to sterilization.3.The expression of Bax,Bak in spermatogenic cells in UU infected rats explained was increased that they are the important factors which promots the cell apoptosis,and mitochondria plays a key role in the regulation to spermatogenic cells.4.Zhi-Bai-Di-Huang Decotion can decrease the expression of Bax and Bak.In other words,one of the mechanisms of Zhi-Bai-Di-Huang Decotion treating UU infection and improving the sperm quality is to anti-apoptosis through regulate the expressions of the apoptosis effect factors Bax and Bak.