On Study Of Relations Of Ureaplasma Urealyticum, Chlamydia Trachomatis And Male Infertility

According to WHO, infertility affects approximately 15 % of couples trying to conceive and a male cause is believed to be a sole or contributing factor in approximately 40% of these cases. Currently, male infertility has been a world matter. Many factors are responsible for male infertility, including biological factors, microbe infection, and so on.Infection of exoteric microbe could disturb transportation of sperm and affect male eugenesis. Ureaplasma urealyticum and Chlamydia trachomatis were known, because those infections affect the male fertility. Ureaplasma urealyticum and Chlamydia trachomatis can make semen parameters decreased, but its mechinanism are not known for us.Sperm density is a cause of male infertility. According to WHO, if sperm density is lower than 20×106, it is oligospermatism. Sperm morphology may be considered as a marker for clinical evaluation of infertility in males. Now, this variable still is helpful when counselling patients before they make the decision to proceed with in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI)-ET. It has been showed that sperm morphology analysis using strict criteria as a prognostic factor in intrauterine insemination. Some researchers reported that there is a direct relationship between sperm morphology and pregnancy rate. Accurate evaluation of normal sperm morphology results should be an integral part of evaluating the male factor. Sperm density, sperm vitality and sperm morphology are very important parameters for evaluation of male infertility. They can represent oligospermatism,asthenospermia teratozoospermia, respectively.In addition, semen leukocyte, antisperm antibody can directly result in male infertility, and indirectly influence male infertility by affecting semen parameters. Ureaplasma urealyticum and Chlamydia trachomatis could be bound with production of semen leukocyte and antisperm antibody. Therefore, Ureaplasma urealyticum and Chlamydia trachomatis can indirectly influence male infertility by producing semen leukocyte and antisperm antibody.In the current study, several comparative studies were carried out to clarify whether Ureaplasma urealyticum and Chlamydia trachomatis influence on semen parameters or not, and to study relationship of between UU, CT and semen leukocyte, antisperm antibody. Semen specimens were obtained form 394 subjects of male infertility. The sperm morphology analysis using semiautomated sperm morphology analyzer (ASMA) was performed. To analyze Ureaplasma urealyticum, the culture method was used. To analyze Chlamydia trachomatis, enzyme method was used. ELISA method was also used in measuring antisperm anbody. They were measured by corresponding reagent kit. To analyze leukocyte number of semen, the bengidine staining method was used. Results:1. Sperm density, viability, vitility of UU positive group were significantly lower than that of fertile group and UU negative group (P<0.01). The percentage of morphologically normal sperm of UU positive group were significantly lower than that of fertile group and UU negative group (P<0.05). The percentage of head defect, tail defect of UU positive group were significantly higher than that of fertile group and UU negative group (P<0.05).In sperm motility parameters, VCL, VSL, VAP, ALH, LIN of UU positive group were significantly lower than that of fertile group and UU negative group (P<0.01). But, BCF of UU positive group were significantly higher than that of fertile group (P<0.01). In UU positive group, the percentage of abnormal semen liquation is 31.7%. In UU negative group, it is 7.3%. The former was statistics difference in comparison with the later(P<0.01).Semen leukocyte of UU positive group were significantly higher than that of fertile group and UU negative group (P<0.001). In UU positive group, the percentage of antisperm antibody positive is 73.4%. In UU negative group, it is 16.1%. The former was statistics difference in comparison with the later(P<0.01).2. Sperm density, viability, vitility of CT positive group were significantly lower than that of fertile group and CT negative group (P<0.01). The percentage of morphologically normal sperm of CT positive group were significantly lower than that of fertile group and CT negative group (P<0.001, P<0.05). The percentage of head defect, tail defect of CT positive group were significantly higher than that of fertile group and CT negative group (P<0.005).In sperm motility parameters, VCL, VSL, VAP, MAD, LIN, STR of CT positive group were significantly lower than that of fertile group (P<0.01). VCL, VSL, VAP of CT positive group were significantly lower than that of CT negative group (P<0.01). In CT positive group, the percentage of abnormal semen liquation is 28.9%. In CT negative group, it is 7.1%. The former was statistics difference in comparison with the later(P<0.01).Semen leukocyte of CT positive group were significantly higher than that of fertile group and CT negative group (P<0.001). In CT positive group, the percentage of antisperm antibody positive is 64.5%. In CT negative group, it is 14.3%. The former was statistics difference in comparison with the later(P<0.01).All results showed that infect of UU, CT make semen parameters decreased. Infect of UU, CT can stimulate the production of semen leukocyte and antisperm antibody. Ureaplasma urealyticum and Chlamydia trachomatis can affect male infertility form different direct and indirect pathway. It was also showed that infect of UU, CT might be considered as a marker for clinical evaluation of infertility in males.