Expression And Clinicopathological Significance Of CEACAM6, MMP-9 In Human Gastric Carcinoma

Objective:To investigate the role and significance in development and angiogenesis of gastric cancer by analyzing the association between angiogenesis and clinicopathological characteristics of gastric cancer and expression of carcinoembryonic antigen-related cell adhesion molecule (CEACAM6) and matrix metalloproteinase 9 (MMP-9) in human gastric carcinoma tissue, adjacent normal tissues and incisal margin normal tissues.Methods:Immunohistochemistry (Envision two-step) was used to detect the expression of CEACAM6 and MMP-9 in 54 cases of gastric cancer and adjacent normal tissues and 20 cases of incisal margin normal tissues. Microvessel density (MVD) was also detected by immunohistochemistry by Anti-factorⅧmarker.Results:1. The positive rate of CEACAM6 in gastric carcinoma tissue (85.19%) was significantly higher than those in adjacent normal tissue (40.74%) and incisal margin normal tissues (15%) (P< 0.05). The expression of CEACAM6 was associated significantly with depth of invasion and clinical stage of gastric cancer (P<0.05), but it was not associated with gender, age, histological type, tumor location, degree of differentiation and lymphnode metastasis (P>0.05).2. The positive rate of MMP-9 in gastric carcinoma tissue (74.07%) was significantly higher than those in adjacent normal tissue (33.33%) and incisal margin normal tissues (10%) (P<0.05). The expression of MMP-9 was associated significantly with tumor location, degree of differentiation, lymphnode metastasis, depth of invasion and clinical stage of gastric cancer (P<0.05), but it was not associated with gender, age and histological type (P>0.05).3. The value of MVD in gastric carcinoma tissue (32.17±8.97) was significantly higher than those in adjacent normal tissue (25.05±6.19) and incisal margin normal tissues (18.62±5.07) (P<0.05). The value of MVD was associated significantly with histological type, tumor location, degree of differentiation, lymphnode metastasis, depth of invasion and clinical stage of gastric cancer (P<0.05), but it was not associated with gender and age (P>0.05).4. There was positive correlation between CEACAM6 and MMP-9 proteins expression in gastric carcinoma tissue (r=0.593, P=0.000).5. The values of MVD in the group of positive expression of CEACAM6 and MMP-9 were higher than those in the group of negative expression of them respectively. The values of MVD in group of CEACAM6 and MMP-9 positive expression (33.16±9.38) were higher than in CEACAM6 and MMP-9 negative expression group (15.07±9.31) and CEACAM6 positive and MMP-9 negative expression group (22.95±3.99) (P<0.05).Conclusions:1. High expression of CEACAM6 and MMP-9 was associated with clinical stage, invasion and metastasis of gastric cancer.2. CEACAM6 and MMP-9 jointly contributed to carcinogenesis and development of gastric cancer.3. CEACAM6 and MMP-9 jointly contributed to tumor micro-angiogenesis of gastric cancer, and there may be synergistic effects of the both for angiogenesis of gastric cancer.