Clinical Study Of Cluster Needling Of Scalp Point Therapy Combined With Rehabilitation Training On The Ability Of Learing Andmemory Of Newborn Rat With Hypoxic-Ischemic Brain Damage

Objective:To observe the effect of cluster needling of scalp point(CNSP) and rehabilitation on ability of learning and memory in newborn rat with hypoxic-ischemic brain damage.Method:To divided 90 healthy SD newbprn rats (7 days old) into 3 groups randomly:model group, CNSP group, CNSP combine rehabilitation group, each with 10 rats. Then divided each group into A,B,C three group equally.After operation the model group feeded normally with no treatment. CNSP group received cluster needling of scalp point 24h after operation.The CNSP combine rehabilitation group received scalp point needling and swimming Training.Using immunohistochemical technique to observe the expression of synaptophysin in area CA1 of the rat hippocampus on 7th day and 14th day.Test the ability of learning and memory of C group in Morris Water Maze.Result:( 1) Behavior test showed that Compared with the model group, CNSP combine rehabilitation group have great significant difference in time using,error frequency and repetitions in Morris Water Maze (P<0.01 ); Compared with the model group,all indexes of CNSP group have significant difference (P<0.05 ); Compared with the CNSP group,all indexes of CNSP combine rehabilitation group have significant difference (P<0.05 );( 2)Compared with the model group the expression of synaptophysin of CNSP combine rehabilitation group have great significant difference on 7th day and 14th day (P<0.01 ); Compared with the model group the expression of synaptophysin of CNSP group have significant difference on 7th day and 14th day (P<0.05 ); Compared with the CNSP group the expression of synaptophysin of CNSP combine rehabilitation group have significant difference on 7th day and 14th day (P<0.05 ); On 14th day the expression of synaptophysin were much better than the 7th day.Conclusion:CNSP combine rehabilitation treatment can improve the ability of learning and memory in newborn rat with hypoxic-ischemic brain damage. Provide referred theoretical basis for adopting the CNSP combine rehabilitation therapy treats cerebral infarction in clinic.