The Efficiency Of Scalp Cluster-needling On Chronic Cerebral Ischemia Big Rats' Learning And Memory Ability, Hif-ia And Its Target Gene's Vegf Expression

Objective:To discuss the mechanism of scalp cluster-needling on chronic cerebral ischemia big rats, by observing the efficiency of learning and memory ability, HIF-1α and VEGF expression. The objective is to provide theoretical basis of treating chronic cerebral ischemia by scalp cluster-needling on clinical.Methods:72wistar big rats with normal learning and memory ability which were chosen by Morris water maze, were randomly divided into a control group (Group A), a sham operation group (Group B), and an operation group. To establish chronic cerebral ischemia models by modified double common carotid artery permanent ligation for operation group. Determined the big rats' learning and memory ability by Morris water maze4weeks after operation, and divided successfully modeled rats into a model group(Group C), a Nimodipine (Group D) and a scalp cluster-needling group group (Group E). No treatment for Group A, B and C. Group D were given Nimodipine1mg/kg by gavage, once daily and4weeks treatment.Group E were given scalp cluster-needling, acupunctured Baihui and both points2mm aside, remained the needles for30min, once daily and4weeks treatment. Determined the big rats'learning and memory ability by Morris water maze8weeks after operation (4weeks after treatment), observed form changes of prefrontal cortex and hippocampus cells by light microscope, determined protein expression of prefrontal cortex, hippocampus HIF-1α,VEGF by Immunohistochemistry and expression of prefrontal cortex, hippocampus HIF-1α,VEGFmRNA were determined by RT-PCR.Results:1. Results of Morris water maze:Learning and memory ability were markedly decreased in Group C, D and E8weeks after operation, with significant differences compared with Group A (P<0.05); there were significant differences in Group D and E, compared with Group C (P<0.05), learning and memory ability in Group E had increased; there were no significant differences between Group D and E.2. Results of hematoxylin-eosin staining:There were no marked pathological changes in Group A and B, neuro cells in Group C were markedly decreased with cells sparsely arranged, seriously deformed, karyopyknosis, cytoplasm disappeared, uneven dye color marks and fuzzy neucleolus. Neuro cells in Group A and B were regularly arranged and formed, with little pycnosis pyramidal cell. Brain tissue damages were markedly reduced compared with Group C3. Results of immunohistochemical staining on HIF-1α,VEGF: Neuro cells in Group A and B were regular with a little positive cells express of HIF-1α,VEGF, there were no significant differences btween Group A and B (P>0.05); HIF-1α,VEGF expression in Group C and D were increased with big body, polygon and different stainings with significant differences compared with Group A and B (P<0.05); there were higher expressions in Group D. and E compared with Group C with significant differences (P<0.05). Positive expression rates in Group E were higher than in Group D with significant differences (p<0.05).4. Results of HIF-1α,VEGF determined by RT-PCR:Expression in Group A and B were inhibited with no significant differences compared within the two groups (P>0.05); there were amplified band of HIF-1α,VEGF amRNA in Group C, D and E, with higher expression abundance compared with Group A with significant differences (P<0.05); Expressions in Group D and E were higher compared with Group C with significant differences (P<0.05); expressions in Group E were higher than in group D with significant differences (P<0.05)Conclusion:1.Chronic cerebral ischemia models established by modified double common carotid artery permanent ligation, and commented by behaviouristics (Morris water maze) and histology (HE staining), were successful and reliable.2.Scalp cluster-needling can improve chronic cerebral ischemia big rats'learning and memory ability by improving pathological changes of prefrontal cortex and hippocampus tissue.3.Scalp cluster-needling can up-regulate expression of HIF-la and VEGF, promote regeneration of vesscular and neuron to protect the brain.4.Scalp cluster-needling can be used on pretreatment of learning and memorizing disorder caused by chronic cerebral ischemia, and is expected to be a new therapy.