The Association Between Pigment Epithelium-Derived Factor And Urinary Albumin Excretion Rate In Type 2 Diabetes Mellitus

ObjectiveTo investigate the relationship between the level of pigment epithelium-derived factor (PEDF) in serum and urinary albumin excretion rate in type 2 diabetes mellitus (T2DM), explore the role of PEDF in the development of diabetic nephropathy and to provide the clue and evidence of early diagnosis and treatment in diabetic nephropathy.MethodAccording to the criterion,30 healthy persons (male 15, female 15) that got checkup in Qingdao Municipal Hospital medical checkup center and 90 inpatients (male 44, female 46) in the Department of Endocrinology of Qingdao Municipal Hospital from June 2009 to December 2009 were divided into three groups and 120 cases in total for subjects. Groups were healthy control group, T2DM normal albuminuria group, T2DM microalbuminuria group and T2DM macroalbuminuria group,30 persons in each group. Blood pressure was measured, BMI was calculated, blood were taken to detect FPG, FCP, CHO, TG, LDL, HDL and HbA1c,24h urine was taken for UAER detection. PEDF levels in patient blood serum were assayed by ELISA.Results①There were no significant differences between healthy control group, T2DM normal albuminuria group, T2DM microalbuminuria group, T2DM group macroalbuminuria group in the factors of age and sex (P> 0.05). The blood serum PEDF levels were increased from the normal control group, T2DM normal protein urinary Group, T2DM microalbuminuria group to T2DM macroalbuminuria group (4.86±1.22μg/ml,6.59±1.29μg/ml, 7.93±1.84μg/ml,10.17±2.27μg/ml), there were significant differences between groups (P <0.05).②PEDF was positively correlated with UAER (r=0.482, P<0.01), SBP (r=0.377, P<0.01), DBP (r=0.331, P<0.01), BMI (r=0.377, P<0.01), FPG (r=0.552, P<0.01), HbA1c (r=0.581, P<0.01), CHO (r=0.478, P<0.01), TG (r=0.371, P<0.01), LDL (r=0.418 P<0.01), DBP (r=0.331, P<0.01), BMI (r=0.377, P<0.01), FPG (r=0.552, P<0.01), HbA1c (r=0.581, P<0.01), CHO (r=0.478, P<0.01), TG (r=0.371, P<0.01), LDL (r=0.418 P<0.01), negatively correlated with FCP (r=-0.565, P<0.01), HDL(r=-0.321, P<0.01) and no obviously correlation.with age and gender (P>0.05).③HbA1c (β=0.261, P<0.01), CHO (β=0.242, P<0.05), UAER (β=0.175, P<0.01), HDL (β=-0.191, P<0.01), BMI (β=0.182 P<0.01) were the independent factors of the serum PEDF level by stepwise multiple linear regression analysis.ConclusionsPEDF levels in T2DM patients are significantly increased and increase gradually with the development of the severity of albuminuria. HbA1c, CHO, UAER, HDL and BMI are the independent factors of the serum PEDF levels. Elevated serum levels of PEDF may be involved in the occurrence and development of diabetic nephropathy to play some compensatory protection. Serum levels of PEDF may predict a sensitive indicator of occurrence and development in diabetic nephropathy...