Expression Of Human Beta-defensin-1 And Dendritic Cells Infiltrating And Correlations In Human Non-small Cell Lung Cancer

Background and Objective:Lung cancer is one of the most common malignant tumors with poor prognosis. Although the mechanism remains unclear, increasing evidence show that human beta-defensin 1(HBD-1), regarded as one kind of endogenous antimicrobial peptide and chemokine, is highly expressed on kideney, abdominal salivary gland, anterior chamber of the eye, nasal mucosa,femal genital tract on mRNA level and protein level. In lung cancer the mRNA expression of HBD-1 is high, while the protein expression is not known yet. HBD-1 directly takes part in reactions of anti-microorganism and adjustability of immunity modulation, may also affects the immunologic reaction in tumors.Thus, further studies are required to determine the function of HBD-1 in lung cancer. In this study we will focus on the the relationship between HBD-1, dendritic cells infiltrating and non-small cell lung cancer immunity.Methods:58 lung cancer tissues samples were collected by pathologic tissues center of Taizhou hospital from Feb 2008 to Feb 2010. All patients did not receive any chemotherapy or radiotherapy before surgeries. These samples included adenocarcinoma (29 cases), squamous cell carcinoma (24 cases) and bronchoalveolar carcinoma (5 cases). while by TNM staging there were 16 cases at stage I,25 cases at stage II and 17 cases at stageâ…¢. Each sample contained tumor tissue and normal tissue. We respectively detected (1) the positive expression rate of HBD-1 in these two parts by immunohistochemistry(EnVision), and then analyzed the data according to pathologic type and TNM stage; (2)the density of dendritic cells infiltrating in each sample by EnVision using protein S100 as DC-specific label marker, and then analyzed the correlation between HBD-1 expression and DC infiltration in the lung cancer tissues.Results:(1) The expression of HBD-1 in non-small cell lung cancer was enhanced significantly(P=0.000), compared to that in normal tissues. The positive expression rates of HBD-1 in tumor was 70.1%, while in normal tissues it was only 20.7%. (2) The expression of HBD-1 in adenocarcinoma was enhanced significantly, compared to squamous cell carcinoma that in (P<0.001). The positive rate of HBD-1 expression in adenocarcinoma and squamous cell carcinoma was 86.2% and 50.0%,respectively. (3) The expression of HBD-1 in lung cancer tissues was similar amongâ… ,â…¡andâ…¢TNM stage,the positive expression rate of HBD-1 was 50.0% at stageâ… ,72% at stageâ…¡, 70.6% at sageâ…¢, compared to each other, stageâ… and stageâ…¡, stageâ…¡and stageâ…¢, stageâ… and stageâ…¢, P values were 0.123,0.987,0.119, there were no significant differences. (4) The positive rate of S100-labeled DCs in tumor issues was significantly increased, compared to the rate in normal tissue, the positive expression rate of S100-labeled DCs in tumors was 77.6%, while in normal tissues it was only 15.5%(P<0.05). (5) There was significant correlation between HBD-1 expression and DC infiltration in the tumor tissues by using spearman correlation analysis (P<0.05). Conclusion:The data shows that HBD-1 was higher expressed in the non-small cell lung cancer tissues, and the expression level was positively correlated with DC infiltration, suggesting that HBD-1 may play an important role in anti-tumor immunity through aggregating immature DCs in tumor tissues.