Correlation Of NOD2/CARD15 And OCTN Gene Polymorphisms And Genetic Susceptility To Inflammatory Bowel Disease In Zhuang Population In Guangxi Province

Objective To explore the relationship between NOD2/CARD15 gene R702W, G908R and L1007fs, OCTN1 gene C1672T and OCNT2 gene G-207C polymorphisms and genetic susceptibility to inflammatory bowel disease (IBD) in Zhuang population of Guangxi province.Methods Intestinal tissue samples of seventy Zhuang and seventy-six Han unrelated IBD patients and eighty Zhuang and eighty-four Han unrelated healthy controls were collected in Guangxi district from Feb,2007 to Oct,2010. Genomic DNA was obtained from intestinal tissues by phenol chloroform extraction method. The polymorphisms of NOD2/CARD15 gene R702W, G908R and L1007fs, OCTN1 gene C1672T and OCTN2 gene G-207C were detected by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).Results Mutational genotypes of the NOD2/CARD15 gene R702W, G908R and L1007fs, OCTN2 gene C1672T and OCTN2 gene G-207C polymorphisms was not found between IBD patients and healthy controls. There was no statistical significance of the genotype frequency and the allele frequency of above SNPs site between IBD patients and healthy controls in Zhuang and Han population of Guangxi province (P＞0.05).Conclusion NOD2/CARD15 gene R702W, G908R and L1007fs,OCTN1 gene C1672T and OCTN2 gene G-207C polymorphisms are not associated with IBD in Zhuang population of Guangxi province.