Protective Effect Of YiganZhuanyin Granules On Acute Chemical Liver Injury In Mice

Objective: To observe the protective effect of Yiganzhuanyin Granules (YGZYDG) on acute chemical liver injury in mice. Methods: Two methods of setting chemical-induced liver injury mice models by separately using subcutaneous injection of CCl₄ and intraperitoneal injection of D-GalN Kunming mice were randomly divided into NC group, liver-injured group, high-, medium- and low-dose of YGZYDG groups (0.4, 0.2 and 0.1 g?kg-1 respectively), and biphenyldicarboxylate (BPDC) group. The indexes of liver, spleen and thymus were observed. The activities of ALT, AST and AKP, the ability of T-AOC in serum, the content of Alb; the content of MDA, and the activity of SOD in liver tissue were investigated. Hematoxylineosin (HE) stain was used to examine the degree of hepatic injury. Results: Compared with model group, YGZYDG had no effect on the weight of spleen, but could obviously reduce the weights of liver and thymus; The activities of ALT, AST and AKP, and the content of MDA could be decreased; the ability of T-AOC, the content of Alb, and the activity of SOD could be increased (P<0.01 or P<0.05). The degree of hepatic injury could be lessened. Conclusion: YGZYDG has protective effect on acute chemical liver injury. The mechanism may be related to attenuating free radical and inhibiting the effect on lipid peroxidation. Objective: To study the effect of YGZYDG against CCl₄-induced liver fibrosis in rats and its mechanism.Methods: Wistar rats were divided into two groups randomly: hepatic fibrosis model group and normal control group.The rats of Hepatic fibrosis model group were established by intragastric administration (i.g.) of 50% CCl₄ for 8 weeks, and the rats in the normal control group, normal saline (NS) were given too. 50 Wistar rats of hepatic fibrosis group that confirmed by the pathological inspection were divided into 5 groups randomly. All rats were treated with drugs or NS for five consecutive weeks by i.g., one time a day. 24 hours after the last administration of drugs, all rats were killed.(1) The blood serum and hepatic tissue were taken, and the activities of ALT, AST in serum and the contents of Hyp, SOD, MDA, GSH-PX in hepatic tissue were measured. The indexes of liver, spleen and thymus were observed.(2) Pieces of hepatic tissues in the identical spot of liver, size 1 cm×1 cm×l cm, were taken, were soaked in 4% paraformaldehyde solution. Hematoxylineosin (HE) stain was used to examine the degree of hepatic fibrosis in rats.(3) The expressions of Col-I, TIMP-1and TGF-β1 mRNA in hepatic tissue were determined by RT-PCR.Results:(1) Compared with model group, YGZYDG could obviously reduce the weights of liver and thymus, but had no effect on the weight of spleen.(2) Compared with the model control group, the activity of ALT and AST in serum was decreased significantly in all YGZYDG groups and positive control group (P <0.01); the contents of SOD,GSH-PX in hepatic tissue were increased significantly in all YGZYDG groups and positive control group (P<0.05 or P<0.01); and the contents of Hyp,MDA were decreased significantly in the meantime (P<0.05 or P<0.01).(3) The pathological results of HE staining showed that, compared with the model control group, the rank of hepatic fibrosis in rats of YGZYDGH, YGZYDGM,YGZYDGL and colchicines was significantly lower (P<0.05 or P<0.01).(4) The expressions of TIMP-1 and TGF-β1 mRNA were down-regulated by all dose of YGZYDG(P<0.01); Col-I mRNA were down-regulated by YGZYDGH, YGZYDGM and colchicine (P<0.01).Conclusion: YGZYDG might have certain curative effect on CCl₄-induced liver fibrosis in rats,its mechanism may be related to attenuating free radical, inhibiting the lipid peroxidation, reducing the collagen synthesis and the deposition, decreasing the sedimentation of ECM and increasing the degradation of ECM. Objective:To investigate the effects and mechanism of YGZYDG and its drug serum on the proliferation, related gene of HSC-T6. Method:HSC-T6 was cultured in vitro, and MTT assay was used to evaluate the inhibitive effect of YGZYDG. and the expressions of Col-I, TIMP-1 and TGF-β1 mRNA were determined by RT-PCR. Result : YGZYDG could obviously inhibit the proliferation of HSC-T6; the expressions of Col-I, TIMP-1 and TGF-β1 mRNA were down-regulated by each concentration of YGZYDG (P<0.01); TIMP-1, TGF-β1 mRNA were down-regulated by each concentration of YGZYDGS (P<0.01); Col-I mRNA was up-regulated by YGZYDGSH, YGZYDGSM (P<0.05 or P<0.01). Conclusion:YGZYDG could inhibit the proliferation of HSC-T6, reduce the collagen synthesis, decrease the sedimentation of ECM and increase the degradation of ECM, it might be one of its mechanism of anti-hepatic fibrosis.