The Effect Of Basic Fibroblast Growth Factor On The Content Of Syndecan-4 By Human Periodontal Ligament Cells

The Effect of Basic Fibroblast Growth Factor on The Content of syndecan-4 by human Periodontal Ligament CellsPeriodontitis is a kind of infection-based diseases which the main is periodontal tissue destruction. The ultimate goal of periodontal therapy not only to eliminate pathogenic factors and to control the development of the disease,more importantly, recovery the destruction of periodontal tissue structure and function. The periodontal tissue regeneration is the most ideal of periodontal treatment. PDLC is a major cellular components of periodontal membrane and is the seed cells of periodontal tissue regeneration. It has multiplex differentiation potential, can differentiated into many cells in specific condition, is the precursor cells in periodontal tissues. A certain number of healthy periodontal ligament cells are thebasis for periodontal tissue regeneration and repair. The adhesion and migration of PDLC in the root surface is the key procedure of periodontal tissue regeneration, which is affected by many factors,include cells in their proliferative activity and peptide growth factors on the regulation of cell growth and differentiation. To a certain extent, PDLC proliferation and migration determines the prognosis of treatment.Syndecan-4 is a transmembrane heparan sulfate protein and composed by a core protein and glycosaminoglycan, is widely expressed in mammals and invertebrates, regulating various cell biological function.Syndecan involved in cell behaviorin such as cell extension, recognition, adhesion, migration and proliferation activities, and play an important role in wound healing. As one of proteoglycan on cell surface, syndecan are the FGF receptor, has an indispensable role in the process of interaction with the bFGF.bFGF distributed in many tissues ,has a wide range of biological effects.As an important modulator on cell proliferation and differentiation bFGF is known to induce chemotactic, angiogenic, and mitogenic activity and has a very important significance in early differentiation and tissue injury repair. Via binding of specific transmembrane receptors,the high affinity of FGFs and the low affinity of glycoprotein heparin sulfate (HSPG), bFGF activates the subsequent intracellular second messenger cascades and leads to diverse biological effects. Heparin sulfate chains of syndecan as the signal transduction mechanism of bFGF, biological effect of bFGF under the control of Syndecan-4. bFGF should first linked with heparan sulfate part of the extracellular domain of Syndecan-4,then combined with the high-affinity receptors play a promoting cell proliferation, migration and differentiation.Objective: To study the content of syndecan-4 in the culture supernatant of PDLC induced by bFGF, combined with previous research, explore the effect and mechanism of bFGF on periodontal tissue regeneration and.Methods:The 4th generation of human PDLC which is freezing were recovery and culture, stimulated PDLC with different concentrations of bFGF, without bFGF as a negative control group. The content of syndecan-4 in the culture medium of human PDLC induced by bFGF was assessed by ELISA enzyme-linked immunosorbent assay.Results: (1.0ng/ml, 10 ng / ml) bFGF can increase the content of syndecan-4 in culture medium supernatant ,the promoting effect of 10ng/ml is more significant, but the effect then decreased in 48h. the concentration of bFGF may affect the decreas trend,the trend of 1.0 ng/ml is weaker than 10 ng/ml.Conclusion: bFGF increase the content of syndecan-4 in culture medium supernatant of PDLC,the promoting effect of 10ng/ml is more significant, the effect were relevant with induced time and concentration of bFGF to some extent.In short, Periodontal tissue regeneration is a very complex process. the specific role of the migration and proliferation processes are not very clear. Combined with our previous experimental study result ,Our results confirm that bFGF promote the PDLC proliferation, which may be regulated by the mechanism that inhibition the syndecan-4 gene expression on PDLC and promote the shedding of extracellular region. Therefore, it is necessary to study the mechanism of interation with bFGF and sydecan-4 in detail, which could provide a heoretical basis for periodontal regeneration...