The Experimental Research About The Mechanism Of The Mulberry Fruit Inhibitint The Balance Of Matrix Metalloproteinase-13 On The Therapy Of Tumor

Tumor invasion and metastasis is a primary deadly cause, the rate of tumor incidence and mortality increase year by year, which becomes more and more distinct. The course of tumor invasion and metastasis is complicated, therefore, we don't only research the metastasis of microcosmic course, but also exploit new medicine that can inhibit the tumor metastasis. Metastasis of the malignant tumor is the pathology basic biological characteristics, which is the main cause of the majority of cancer patients leading to treatment failure. For the past few years, person pay more attention to the tumor metastasis. The course of tumor invasion and metastasis is a process of which primary tumor cells transfer to surrounding or distant tissues, involving tumor cells through extracellular matrix barriers, vascular basement membrane wall and entering into the host micro-environment.It has been found that the capacity of invasion and metastasis of tumor cells is positive correlation to protease degrading its extracellular matrix, basement membrane. Matrix metallo-proteinases can degrade almost all ECM ingredients, which is the propulsion to tumor invasion and metastasis. MMP is promoter action in tumor invasion and metastasis, therefore inhibitors of MMPs is also paid much attention to. Matrix metalloproteinase inhibitors (TIMPs) can inhibit the hydrolysis activity of MMPs, can inhibit the role of angiogenesis in tumor treatment, which is a lighspot in oncother apy. At present, there is not enough evidence to prove whether the surgery, radiotherapy and chemotherapy treatment can control the tumor metastasis, while traditional Chinese medicine has unique advantages in improving quality of life of cancer patients, extending the survival time of patients with tumors. There are the following mechanisms in Traditional Chinese medicine: Firstly, six evils, generally speaking, including some chemical and physical factors invading the human body and influencing the run of Qi and blood leads to tumor. Secondly, anxiety, anger and grief lead to the stagnation of Qi, blood and phlegmatic malaria. Thirdly, the factors of the insufficiency of Zang and Fu, the disorder of Qi and blood, exogenous evil, emotion, drink and food can easily hurt the human body.Traditional Chinese medicine have been a most important way of anticancer, and a single drug of traditiongal Chinese medicine have been carried out. Sapor of clove is pungent and lukewarm, go back to spleen, stomach, lungs, kidney. The efficacy of warming middle energizer descend adverse-rising and reinforce the kidney yang is master. In clinical practice, the effect of clove is very broad. It can treat hiccup, vomit, regurgitation, Xie Li, chest and abdomen paint, and so on. The modern research achievements make known that, hempseed has obvious therapeutic effect to the liver, stomach, cervical cancer, we can suppose that its treatment of tumors in vivo may be related to inhibition of matrix metalloproteinase hyper-expression. In this paper, hemp seed treatment study the mechanism of tumor will be studied.Objectives:To observe whether the extract of the clove can inhibit the matrix metalloproteinases, which offer essential theoretical and experimental foundation for the mechanism of the hempseed inhibiting tumor.Methods:Put a mount of MMP-13 solution to the enzyme buffer solution and the hempseed solution of 1.2ug/ml, 1.0ug/ml,0.8ug/ml, 1ug/ml,0.6ug/ml differently, incubate 30 minutes,then add 0.2μg DQ-gelatin and test the substrate.The fluorescence intensity can response the ability of enzyme degrading the substrate. Principles:The fluorescence can be emitted when the enzyme degrades the substrate, therefore testing the fluorescence intensity can response the ability of enzyme degrading the substrate. DQ-gelatin which is signed by the fluorophore is used in the experiment.After the enzyme degrades the substrate, the fluorophore is separated with quenching group And to some extent, fluorescence intensity is proportional to the speed of enzyme degrading substrate.Results:The extract of the clove have the effect of inhibiting MMP-13 and the function depends on the density.That is to say, the hempseed extract on the inhibition of MMP-13 enhances With the increase in drug concentration,which confirms the new drugs extracted from the natural and targeted to MMPs/ TIMPs has bright prospects further.Conclusion:The extract of the clove can inhibit the activity of the MMP-13,and this kind of function depends on the density.