Proteomics Study Of Cerebrospinal Fluid In GBS Patients

Guillain-Barre syndrome (GBS) is a disease with pathological changes of lymphocyte & macrophage infiltration surrounding the small vessels and demyelination of mylin sheath. Its clinical manifestation includes flaccid paralysis of extremities, facial paralysis, sensory dysfunction of peripheral never system and decreased tendon reflex. However, its etiology is not fully known.Objective:Historically, elevation of protein level in cerebrospinal fluid (CSF) is the characteristics of GBS. This typical change provides an unique opportunity to inquire related specific proteins and pathogenesis of GBS by proteomics study. The purpose of this experiment is to determine the related specific proteins of GBS, find new important clue for pathogenesis, and provide theorical evidence and new means of GBS diagnosis and treatment.Methods:(1) Six outbreaking GBS patients of AMAN type from Shuangyang Country in 2007 was chosen into the GBS group (T group). Six headache patients who were outpatients from March to May of 2009 in The First Hospital of Jilin University were chosen into Control group (C group). Pateints of C group should be with normal results of CSF routine-biochemical and cytological examination. Age, sex, and other conditions should be matched with GBS group. (2) Two-dimensional difference gel electrophoresis (2-D DIGE), in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), was used to fully analyze CSF of outbreaking GBS patients of AMAN type.Results:(1) Compared to Control group, Antithrombin-â…¢(AT-â…¢) expression up-regulated obviously in GBS patient's CSF in 1.52 times with significant difference (P<0.01). Among it, This the first time to report the differential expression of AT-â…¢in CSF of GBS patients; (2) Compared to Control group, Fibrinogen (Fg) expression down-regulated obviously in GBS patient's CSF in 1.40 times with significant difference (P<0.01); (3) Compared to Control group, ApolipoproteinA-â… (ApoA-â… ) expression up-regulated obviously in GBS patient's CSF in 1.84 times with significant difference (P<0.01); (4) Compared to Control group, Zinc-alpha2-glycoprotein (ZAG) expression up-regulated in GBS patient's CSF in 1.88 times with significant difference (P<0.01); (5) Compared to Control group, Protein 2675 expression down-regulated in GBS patient's CSF in 1.22 times with satatistical difference (p<0.05).Conclusion:(1) Up-regulation of AT-â…¢and down-regulation of Fg in CSF of outbreaking GBS patients indicate that AT-â…¢and Fg may possibly involve in the process of GBS's development, and may play key roles in the pathogencsis of GBS. Among it, the differential expression of AT-â…¢is the first-time to be reported; (2) Up-regulation of ZAG and ApoA-â… in CSF of outbreaking GBS patients imply that ZAG and ApoA-â… may take part in the process of GBS's development, but further confirmation is needed for the particular mechanism.