The Expression Of Survivin And Ki67 In Esophageal Squamous Cell Carcinoma And Their Significance

Esophageal cancer is a common gastrointestinal tumor. Its low early diagnosis rate has led to a large proportion of esophageal cancer detected in mid and late stage, resulting in poor treatment outcome. Thus, the key to its treatment is early detection, which requires highly specific biological marker.Anti-apoptotic protein survivin plays a dual role in apoptosis and cell division. Recent studies have found close relation between survivin and differentiation of esophageal squamous cell carcinoma as well as infiltration of tumor cells. A high survivin expression is also proposed in suppressed apoptosis and in metastasis. Using immunohistochemical methods, the present study demonstrated the relation of cellular differentiation status and invasion depth to survivin expression in esophageal squamous cell carcinoma. It was also hoped that the results of this study would strengthen current body of evidence for specific biological marker in detection of early esophageal cancer and esophageal cancer differentiation. Ki-67 is a more specific cell proliferation antigen.1. The levels of expression of surviving and Ki-67 were detected using immunohistochemical SP from 40 samples of esophageal squamous cell carcinoma and 20 samples of adjacent tissues.2. Using immune chemical methods, the levels of expression of surviving in normal esophageal epithelial cells (Het-IA), well-differentiated esophageal squamous epithelial cells (KYSE-70) and poorly differentiated squamous cell epithelial cells (KYSE-450).3. Statistical analysis, continuity correction and X2 test, were performed using SPSS 13.0. (P≤0.05 was designated to be statistically significant).1. Expression of survivin, ki-67 in esophageal squamous cell carcinoma and adjacent tissuesEsophageal cancer survivin, ki-67 gene expression was significantly different from the adjacent tissue (P<0.05).2. Survivin, Ki-67 in esophageal squamous cell carcinoma and the clinical pathology Statistically significantly difference in Survivin and Ki-67 expressions were found in tumours of different invasion depth and different differential status (p<0.05).Levels of Survivin and Ki-67 expressions were not found to be influenced by other pathological characteristics.3. Expression of Survivin and Ki-67 in esophageal squamous cell carcinoma Spearman rank correlation method of test analysis showed a positive correlation between the expression of Survivin and Ki-67 protein in esophageal squamous cell carcinoma (P<0.05).4. Survivin expression in KRSE-70 cells, KYSE-450 cells and Het-IA cells were 85%,53.8% and 5.3% respectively. Statistically significant difference in the levels of Survivin expression were found in abovementioned cell lines of different differentiatial status (P<0.01).The expression of Survivin and Ki-67 in squamous cell carcinoma was significantly higher than that in normal tissue. The levels of expression were found to have positive correlation with the status of differentiation and invasion, while age, gender and lymph node appeared to have minimal relation to the expression.