Study On Hydrolysis Of Extract Of Pollen Typhae Caudaline To Prepare Isorhamnetin By Hydrochloric Acid

Objective Study on the extraction and purification of total flavones,which are the major active components of Pollen Typhae Caudaline, so as to obtain high purity products. Furthermore, Study on hydrolysis of extract of Pollen Typhae Caudaline to prepare isorhamnetin by hydrochloric acid in order to get flavone aglycone.Methods Using the content of total flavones and extracting ratio as evaluating indexs,we selected the optimum extracting condition by orthogonal; purify the extract of Pollen Typhae Caudaline in order to get a higher purity product, and total flavones in the extract were investigated by visible spectrophotometry. using High Performance Liquid Chromatography (HPLC) determination, visible spectrophotometry and methods of inspection. Results The optimum extracting condition was:50% ethanol immersion 0.5 hours,heating to extract two times,adding alcohol for 10,8 times and extracting for 1.5,1.0 hours. The technological conditions of adsorption and desorption for Radix Wikstroemiae Indicae were as follows:the concentration of extract 1.0g/ml polyphenol sample volume is 1.0 times of the bed volume(BV), and a flow rate of 2 BV/h at room temperature; Diameter and height ratio is 1:3.Then use 4 times BV of distilled water for purification whereas the optimal desorption conditions are found to be a flow rate of 8 BV/h using 5 times BV of 80% ethanol for elution.The acidic hydrolysis conditions are as follows:methanol as medium in which hydrochloric acid concentration20%, ratio of methanol to hydrochloric acid concentration20%(V/V) 4:1, and hydrolyzing for 4 h at 90℃.Conclusion The procedure was reasonable and pratical.