Study On Material Base Of Pollen Typhae And Carbonized Pollen Typhae In Prescription

Pollen Typhae is the dry pollen of Typhae angustifolia Linn.,Typha orientalis Presl or the same genus plant.It has effect of hemostasis by resolving stasis.It is mainly used for varieties of bleeding diseases caused by blood stasis as a common medicine in blood circulation.The effect of carbonized Pollen Typhae was enhanced in hemostasis after being carbonized.At present,Pollen Typhae and carbonized Pollen Typhae is often mixed in clinical applications.The precise effects mechanism of Pollen Typhae and carbonized Pollen Typhae is not clear.To standardize their rational applications in clinic,we carry on the research of material basis related to the different clinical uses of processed and unprocessed Pollen Tyhae,providing a scientific basis for different clinical uses of Pollen Typhae and carbonized Pollen Typhae?The paper is divided into three parts.The first chapter summarized the progression of Pollen Typhae in chemistry,pharmacology and clinical application.The second chapter is Pollen Typhae and carbonized Pollen Typhae's compound of chemical research,including Pollen Typhae and carbonized Pollen Typhae ' s TLC,Shixiao powder of HPLC-MS analysis,Ershen powder of GC-MS and HPLC-MS combined with analysis,HPLC fingerprint of Shixiao powder,Pollen Typhae carbon compound in the dissolution rate of the different ingredients used in the study.The third chapter is promoting blood Pollen Typhae its compound effect evaluation,including the composition of HUVEC protective effect of LPS Pollen Typhae injury,Pollen Typhae,Pollen Typhae charcoal and promoting blood circulation effect Trogopterus Dung compatibility evaluation.Experimental studies are as follows:1 Literature reviewThrough consulting relevant and ancient literature and modern research achievements of scholars both at home and abroad,We have summarized the chemical constituents,phar macological action and clinical application data of Pollen Typhae and carbonized PollenTyphae in this paper.This provides a basis of material basic research for Pollen Typhae's different use in the compound.2 Pollen Typhae and carbonized Pollen Typhae's compound of chemical research.2.1 Pollen Typhae and carbonized Pollen Typhae's TLC.TLC has the dual effect of separation and analysis,greatly improving the isolation and identification of sensitivity and specificity,this paper Pollen Typhae and carbonized Pollen Typhae flavonoid glycoside and aglycone structure,characteristics quite different polarity using two chromatographic conditions qualitative identification,the results showed that after Pollen Typhae fried carbon isorhamnetin-3-O-neohesperidoside,typhaneoside significantly reduced isorhamnetin increase,both glycosides are isorhamnetin aglycone,heated to decomposition of flavonoid glycoside flavonoid aglycone,revealing Pollen Typhae and Pollen Typhae different raw and cooked with charcoal substance of the material basis of the similarities and differences when speculation Pollen Typhae fried carbon.2.2 HPLC-MS analysis of Shixiao powder.Using LC-20A type fast liquid chromatography on Shixiao powder analyzed for major ion fragments cracking process analysis,identified 35 compounds mainly flavonoids and diterpenoids,Pollen Typhae in order to clarify the compound Shixiao powder effects provide the basis for the material basis for the role.2.3 GC-MS analysis and HPLC-MS-MS Analysis of Ershen powder.By GC-MS and LC-20A type fast liquid chromatography to analyze Ershen powder of the major ion fragments cracking process for resolution.Eighteen constituents were identified from Eeshen powder by GC,which accounted for more than 1%in the fomula;and fifteen compounds identified by LC-MS.In LC-MS identified 15 compounds,mainly Cyperus GC charcoal and a small amount of volatile oil components in carbon Pollen Typhae alkanes ingredients,liquidchromatography-mass spectrometry mainly carbon Pollen Typhae flavonoids,provide a basis for clarifying the role of the effect of the material basis carbon Pollen Typhae in the Ershen powder.2.4 HPLC fingerprint of Shixiao powder.This excerpt with 10 batches from different sources Pollen Typhae and Trogopterus Dung,by HPLC,identified five major peaks,including typhaneoside isorhamnetin-3-0-Neohesperidin isorhamnetin,quercetin,Amentotaxus biflavone.Scattered with single herbs through laughing positioning control and UV spectroscopy,the results clearly attributable to the vast majority of peaks,the site is mainly polar Pollen Typhae the ingredients,mainly from small polar Trogopterus Dung.This method is feasible and reproducible,and can be used as fingerprints of compound Shixiao powder.The experimental analysis of the 10 batches Shixiao powder HPLC fingerprint results showed that 10 batches each have 15 peaks,the relative amount of peak area has some differences,similarities were greater than 0.965,the established method can better evaluate quality scattered laugh.2.5 Study composition with different dissolution rates in the compound of Pollen Typhae and carbonized Pollen Typhae.2.5.1 Pollen Typhae replaced carbonized Pollen Typhae impact dissolution rates in the compound of Shixiao powder.This excerpt with Shixiao powder and control Shixiao powder(Shixiao powder in Pollen Typhae replaced Pollen Typhae charcoal),comparing both the amount and the representative extract chemical composition typhaneoside isorhamnetin-3-O-new orange Paper glycosides,flavonoids Amentotaxus double dissolution rate.The results showed that Pollen Typhae and Trogopterus Dung decoction,resulting extract was 14.0%Pollen Typhae Trogopterus Dung alone after boiling with the merger,the resulting extract was 13.8%,the amount of extract improved 0.2%;Shixiao powder in typhaneoside isorhamnetin-3-O-Neohesperidin,Amentotaxus double flavonoid contents were 4.32,3.94,0.0110 mg.g-1,after the merger than the boiling alone compared with 8%,respectively,2%,9%,suggesting Pollen Typhae compatibility with Trogopterus Dung promote dissolution effect.Shixiao powder control together with the decoction compared Shixiao powder combined decoction resulting extract increased 3.3%.The Trogopterus Dung compatibility compared with Pollen Typhae Trogopterus Dung compatibility with Pollen Typhae charcoal,Amentotaxus biflavone content increased by 7%,suggesting that charcoal can promote Pollen Typhae compared Pollen Typhae Trogopterus Dung in Amentotaxus biflavone dissolution.2.5.2 Ershen powder in carbonized Pollen Typhae replaced Pollen Typhae impact on the composition of compound dissolution rates.Ershen powder and control Ershen powder(Ershen powder in Pollen Typhae charcoal replaced Pollen Typhae),comparing both the amount and the representative of the chemical composition of the extract typhaneoside isorhamnetin-3-O-neohesperidin,a-Cyperone dissolution rate.The results showed that Pollen Typhae charcoal and charcoal Cyperus decoction,resulting extract was 9.6%,Pollen Typhae charcoal and charcoal alone Cyperus boiling after the merger,the resulting extract was 9.6%,basically no significant change in the amount of extract;Ershen powder typhaneoside isorhamnetin-3-0-Neohesperidin,?-Cyperone contents were 0.62,0.61,0.39 mg.g-1,compared with a separate comparison boiling after the merger,respectively 12%,10%,6%,and Cyperus presumed Pollen Typhae carbon can facilitate the dissolution of carbon mutual compatibility.Compared with the control Ershen powder together decoction,Ershen powder decoction dissolution rate decreased by 3.2%,should be fried after Pollen Typhae flavonoid glycosides reduce carbon related;Pollen Typhae charcoal and charcoal Cyperus compatibility compared with Cyperus Pollen Typhae carbon compatibility,a-Cyperone content increased by 12%,suggesting that charcoal compared Pollen Typhae Pollen Typhae promote Cyperi charcoal?-Cyperone dissolution.3 Pollen Typhae and promoting blood circulation effect evaluation.3.1 Pollen Typhae the protective effect of composition on HUVEC injury.3.1.1 Pollen Typhae in inhibition of HUVEC value-added components.This section studied the HUVEC cell cultured by MTT colorimetric assay and the relative inhibition rate,the results show that in the appropriate dose range in typhaneoside Pollen Typhae isorhamnetin-3-0-neohesperidoside,quercetin,naringenin,niacin is not only no inhibition of HUVEC value-added,on the contrary there is a certain role in promoting cell proliferation,but showed some activity.Other ingredients naringenin,thymine,uracil,propionic acid,stearic acid esters and herbs in the dose range value does not exhibit inhibition of HUVEC to determine a safe dose range of each component,each component of the concentration of active research provide the basis for dose.3.1.2 Pollen Typhae the components of LPS damage the protective effect of HUVEC.This section was prepared using LPS-induced HUVEC damage model,cell viability was measured by MTT assay,xanthine oxidase cell culture medium was measured superoxide dismutase(SOD)activity,TBA method for the determination of malondialdehyde(MDA)content,nitrate Determination of nitric oxide reductase(NO)levels,the results show,LPS-induced HUVEC cell supernatant NO,MDA levels were significantly increased SOD activity decreased significantly;Pollen Typhae in typhaneoside,isorhamnetin-3-O-neohesperidoside,quercetin,naringenin,niacin can significantly improve cell viability,cell supernatant NO,MDA content decreased,SOD activity increased by inhibiting the release of NO,inhibition of lipid peroxidation and antioxidant protection of vascular damage in human umbilical vein endothelial cells,cells coordinate endothelial balance between clotting and anti-clotting maintain a balance vasodilation factor and contraction factor,so as to maintain the stability of tension and blood vessels,and thus play a role in promoting blood circulation this study explain Pollen Typhae blood circulation mechanisms at the cellular level.3.2 Pollen Typhae,Pollen Typhae charcoal and promoting blood circulation effect Trogopterus Dung compatibility evaluation.3.2.1 Pollen Typhae,Pollen Typhae Trogopterus Dung compatibility with carbon impact on hemorheology and coagulation of blood stasis model.Pollen Typhae carbon products could significantly reduce compound viscosity and plasma viscosity of whole blood stasis model rats showed improvement in blood circulation,and the raw product is superior to carbon compound products.Both can extend each stage clotting time,reduce fibrin levels,showed some blood effects,and the raw product is superior to carbon compound product compound.3.2.2 Pollen Typhae,Pollen Typhae carbon Trogopterus Dung compatibility with platelet blood stasis rats model.Pollen Typhae Sheng products,charcoal product compound can inhibit platelet aggregation induced by ADP,on TXB2 and 6-keto-PGF1? have certain callback action,show some blood effects,and the raw product is superior to carbon compound product compound.3.2.3 Pollen Typhae,Pollen Typhae carbon Trogopterus Dung compatibility with the tongue,lung,uterus effect on blood stasis model rats.Pollen Typhae Sheng products,carbon products available on Stasis Rats Compound tongue,lung uterine injury play a role in the treatment and recovery,and the raw product is stronger than the carbon compound product compound.Pollen Typhae,carbon products have a certain compatibility with Trogopterus Dung role of blood circulation,the blood circulation activity showed lower blood viscosity,prolonged clotting time,platelet aggregation,and the raw product is stronger than carbon products,which is reflected conduct blood raw,fried increased carbon astringent,blood weakened the traditional theory concocted for raw and cooked Pollen Typhae differences in clinical use to provide a scientific basis.