The Sequential Changes Of Malonydialdehy-ed In Secondary Spinal Cord Injury And Its Significance

ObjectiveHuman spinal cord injury is a serious disability and death of important reasons, often after acute spinal cord injury secondary to a variety of neurological and biochemical changes that increase at secondary spinal cord injury may play an important role. Recently, the participation of free radicals in aspects of secondary spinal cord injury more and more attention. This study was designed to set up HIP rat model of spinal injury on the basis of the dynamic observation period after spinal cord injury malondialdehyde (malonydialdehyed, MDA) content changes in the expression of secondary spinal cord to explore the pathological mechanism of injury.MethodsThe health of 70male SD rats, weight 250±20 grams were randomly divided into normal control group,10 injured group 60, the use of hydrostatic model of rat SCI. Rats in each group, respectively,12 hours after injury,48 hours,5 days,7 days,14 days,21 days, each time through the carotid artery bleeding to death. Using hematoxylin-eosin staining to observe pathological changes in spinal cord MDA Detected:bleeding to death quickly check the reference time-point damage to the spinal cord segment was determined by MDA thiobarbituric acid (TBA) colorimetric. Data using SPSS 13.0 for Windows statistics software. All mean to express X±S, different groups within the reference time-point group were compared using a number of one-way anova. ResultsVisible to the naked eye tissue spinal cord injury in rats with congestive. SCI can be seen under 12～48 hours after injury in partial karyopyknosis neurons, cell body narrow, deep-staining cytoplasm, neurons become red. Injury after 2～5 days, axonal swelling, vacuoles appear, white matter nerve myelin disorder, collapse of the breakdown. Injury 14 days after the 7 marked gliosis, and the ghost phenomenon can be seen satellite cells. Injury after 14～21 days, spinal cord injury thinning necrosis collapse of part of gray matter, showing the formation of cystsThe health of 70 male SD rats, weight 250±20 grams were randomly divided into normal control group,10 injured group 60, the use of hydrostatic model of rat SCI. Rats in each group, respectively,12 hours after injury,48 hours,5 days,7 days,14 days,21 days, each time through the carotid artery bleeding to death. Using hematoxylin-eosin staining to observe pathological changes in spinal cord MDA Detected:bleeding to death quickly check the reference time-point damage to the spinal cord segment was determined by MDA thiobarbituric acid (TBA) colorimetric. Data using SPSS 13.0 for Windows statistics software. All mean to express X±S, different groups within the reference time-point group were compared using a number of one-way anova.DiscussionMDA as a lipid peroxidation end product can be a direct reflection of the level of free radicals in the body by free radicals in the body change and the severity of the attack, and its content reflects the level of free radicals in the body's metabolism changes are the result of free radical damage important cells signs. SCI has found that the content of MDA after the spinal cord early could be a significant increase Paragraph bleeding injury occurs mainly in gray matter, the rapid decline in blood perfusion, ischemic metabolite accumulation, a series of continuing or secondary demyelination of the biochemical changes,3d glial number Organize,5～7d produce glial fibrillary. In this study lipid peroxidation was found at significantly within 7d, so to speed up free radical damage, membrane damage.Under the conditions of this experiment, after spinal cord injury at 12h in MDA rapidly increased, representing an increase of 6d after injury 90%,7d rise to a peak followed when decreased 21 days after injury near normal levels. Injury at 7 days, MDA level and injury time-related linear trend. Free radicals in order to demonstrate the importance of spinal cord injury.ConclusionsAfter spinal cord injury in malondialdehyde (MDA) began to increase expression and malondialdehyde (MDA) expression and secondary spinal cord injury and pathological changes of hypoxia-ischemia is closely related to.