Study On Anti-inflammatory, Antipyretic And Analgesic Effect Of Total Flavones From Lagotis Brevituba Maxim

Objective:Study the anti-inflammatory, antipyretic and analgesic effect of total Flavones from Lagotis brevituba Maxim.Methods:1. The inflammatory models were made by dimethylbenzene-induced edema of mice ear, cotton-pellet granuloma of the rat, egg white-induced swelling of rat paw, and the permeability increase of blood capillary by acetic acid in mice, respectively. The models were used to explore the anti-inflammation of the total flavonoids.2. The antipyretic action was monitored by dried yeast-induced,2, 4-dinitrophenol-induced rat pyrexia and endotoxin induced rabbit pyrexia.3. The analgesic effect was verified by HAc-induced writhing response in mice, Hot-plate test and Formaldehyde induced pain.Results:1. The TFL had significant inhibitory action on swelling of dimethylbenzene-induced edema of mice ear at 100,50 and 25 mg·kg-1; The TFL had significant inhibitory action on the cotton-pellet granuloma of the rat, egg white-induced swelling of rat paw and permeability increase of blood capillary by acetic acid in mice at 100 and 50 mg·kg-12. The TFL had significant inhibitory action on dried yeast-induced,2, 4-dinitrophenol-induced rat pyrexia and endotoxin induced rabbit pyrexia. The antipyretic action of TFL (200mg·kg-1) and Aspirin positive control group both can last 6 hour.3. The mice with TFL high and middle dose groups (200,100mg·kg-1) showed fewer writhes of abdominal cavity, and increased the pain threshold in the hot-plate test.All dose groups of TFL (200,100,500mg·kg-1) can obviously reduceⅠandⅡphases of mice foot licking time.Conclusion:The TFL has significant anti-inflammation, antipyretic and analgesic effect, however the mechanism is worth further study.