Research Of Rabdosia Rubescens (henrsl.)hara Germplasm Resources

Objective: In order to master the law of diversity of rabdosia rubescens [Henrsl.]Hara germplasm,and make the quality and genetic relationship difference of rabdosia rubescens [Henrsl.]Hara between different regions and types clearly,we did some scientific experiments. They can provide scientific basis for rational development and utilize of rabdosia rubescens [Henrsl.]Hara resources in China.Method: Determine the oridonin, ponicidin, rosmarinic acid of rabdosia rubescens [Henrsl.]Hara in different areas by HPLC chromatography. At the same time determine ethanol-soluble extractives, ether-soluble extractives by the method of provision of Pharmacopoeia(05 version); research on printfinger of diterpenoids in rabdosia rubescens [Henrsl.]Hara; study the extraction methods of genomic DNA, optimize the methods of ISSR-PCR, and establish fingerprint of rabdosia rubescens [Henrsl.]Hara.Result:1. The quality assessment of rabdosia rubescens [Henrsl.]Hara in different areas.Determine the oridonin, ponicidin , rosmarinic acid ,ethanol-soluble extractives, ether-soluble extractives of rabdosia rubescens [Henrsl.]Hara in different areas.The results are analyzed by cluster analysis through the software SPSS13.0 . The results show the quality of rabdosia rubescens [Henrsl.]Hara. are obviously different, especially on which have similar geographic distribution, and some which have further geographic distribution but similar habitats have the same quality.2. The research on printfinger of diterpenoids in rabdosia rubescens [Henrsl.]Hara.Study the extraction methods of diterpenoids. Establish fingerprint comparison refering to the HPLC chromatography of 10 batches produced by the Taihang Mountains. Chromatographic condition: Column: phenomsil C18(250×4.6mm,5μm); Detection wavelength: 238nm;Flowrate: 0.8ml/min; Column temperature: 30℃; Mobile phase: methanol: 0.5% phosphoric acid gradient elution: 0-25min methanol (45%-50%); 25-60min methanol (52%).The results show the diterpenoids in Rabdosia rubescens [Henrsl.]Hara.in different area are different.,the diterpenoids in different mutation type are changed.3. Extraction of genomic DNA and molecular markers ISSR of Rabdosia rubescens [Henrsl.]Hara. in different areas.Study the extraction methods of genomic DNA and optimization the methods of ISSR-PCR,and establish fingerprint of rabdosia rubescens [Henrsl.]Hara. Establish the tree of genetic relationships of rabdosia rubescens [Henrsl.]Hara by calculating the genetic distance of rabdosia rubescens [Henrsl.]Hara in different areas and different mutation types.The results have showed rabdosia rubescens [Henrsl.]Hara have obviously different.Conclusion: Combined with the research of quality and diterpenoids, we can make sure the germplasm of rabdosia rubescens [Henrsl.]Hara is in polarization. We should make distinction on clinical, industrial production and research.