Effect Of The Treatment Of Invigorating Spleen And Promoting Blood Regulating QI On Transforning Growth Factor-β1,SMAD2/3 And SMAD7 Of The Chronic Pancreatitis Fibrosis Rats

Objective: Chronic pancreatitis (CP) is the organization and function of the pancreas appeared irreversible sustained damage, which typical pathological representation is fabrosis of pancreatic texture . Based on Preliminary research, our discussion group further observed the influence of the treatment principle of"invigorating spleen and promoting blood by regulating qi"on expression of TGF-β1,Smad2/3 and Smad7 when Chronic Pancreatitis (CP) occurred, to penetrate into discussion about this principle's anti-fibrous mechanism.Method: Our research injected L-Arg into abdomen of the rats to make spleen deficiency rat model, it was a new model which combine diseases and syndromes; Utilizing the techniques, such as immuno-histochemistry, optical microscope, the paper investigated the effect of the regulating factors of texture fibrous TGF-β1and Smad2/3,Smad7 expression changes in Chronic Pancreatitis rats; Meanwhile, observes the intervening effect of"YI TAI Compounded Medication". This paper studied the anti-fibrous significance and mechanism from tissue form and molecular level.Result: 1. The treatment principle of"invigorating spleen and promoting blood by regulating qi"could recover most of the pancreas acinar, reduce fiber constituents in pancreas lobular, accelerate absorption of inflammatory cells, increase body's dissolved ability;2 The treatment principle of"invigorating spleen and promoting blood by regulating qi"could restrain the expression of TGF-β1and Smad2/3,and enhance the role of Smad7 expression. so as to restrain its signal transduction, reduce deposition of ECM and promotion degradation of ECM.Conclusion: The treatment principle of"invigorating spleen and promoting blood by regulating qi"is an effective method of preventing and treating CP,"YI TAI Compounded Medication"on the treatment of this disease by inhibiting the production of TGF-β1 as well as the targeting of blocking its transduction pathway Smad2/3 signal, the signal to enhance the expressio of Smad7, Regulating the formation and degradation of ECM, Inhibit the formation and progress of pancreatic fibrosis YI TAI Compounded Medication has a higher clinical value.