Expression Of BAG-1 Isoforms And VEGF In Colorectal Carcinoma And Their Clinical Significance

ObjectiveTo detect the expression of BAG-1 isoforms and VEGF in the colorectal carcinoma, adenoma and normal tissue, analyze the relation of clinical pathologic indexes and their expression to evaluate their role of the occurance and development in the colorectal adeno carcinoma, analyze their relevance to explore their mutual function.MethodsThe expression of BAG-1 and VEGF in 12 cases of normal colorectal mucosa and 40 cases of colorectal adeno carcinoma tissues was detected with immunohistochemical staining, SABC. The expression of BAG-1 isoforms in 23 cases of normal colorectal mucosa and 17 cases of colorectal carcinoma and 6 cases of colorectal adenoma was detected with western blotting.ResultsThe result of immunochemical mothed: the positive rate of BAG-1 protein in normal colorectal mucosa and in colorectal adeno carcinoma tissues was respectively 3/12(25%), 26/40(65%), the diffenrence is significant(p<0.05). The expression of BAG-1 was correlated to the differentiation degree, lymph node metastasis and Duke's stage. The positive rate of BAG-1 protein in poorly differentiated colorectal carcinoma 100%(12/12) is higher significantly than the positive expression of BAG-1 protein in moderately and well differentiated colorectal carcinoma 50%(14/28)(p<0.05), the positive expression rate of BAG-1 protein in colorectal carcinoma with lymph node metastasis 94.4%(17/18) is higher than the positive expression rate of BAG-1 protein in colorectal carcinoma without lymph node metastasis 40.9%(9/22)(p<0.05); the positive expression rate of BAG-1 protein in Duke's C+D 95.2%(20/21) is higher significantly than the positive expression rate of BAG-1 in Duke's A+B 31.6%(6/19)(p<0.05). The positive expression rate of VEGF protein in normal colorectal mucosa and in colorectal adeno carcinoma tissues was respectively 16.6% (2/12), 65% (26/40), the difference is significant(p < 0.05). The expression of VEGF was related to the differentiation degree, the depth of infiltration, lymph node metastasis and Duke's stage. The positive expression rate of VEGF protein in poorly differentiated colorectal carcinoma 100%(12/12) is significantly higher than the positive expression rate of VEGF protein in moderately and well differentiated colorectal carcinoma 50%(14/28)(p < 0.05); the positive expression rate of VEGF protein in colorectal carcinoma with full thickness infiltrated 57.5%(23/30) is higher significantly than the positive expression rate of VEGF protein in colorectal carcinoma with only muscular layer infiltrated 30%(3/10) (p<0.05). The positive expression rate of VEGF protein in colorectal carcinoma with lymph node metastasis(16/18) is higher significantly than the positive expression rate of VEGF protein in colorectal carcinoma without lymph node metastasis(10/22) (p<0.05); The positive expression rate of VEGF protein in Duke's C+D stage 90.5%(19/21) is higher significantly than the positive expression rate of VEGF protein in Duke's A+B stage 36.8%(7/19) (p<0.05). The expression of BAG-1 and VEGF protein was positive correlated, the correlation coefficient is 0.451 (p<0.05).The result of western blotting method: the isoforms of BAG-1 protein expressed in the colorectal carcinoma is P33(BAG-1S), and the expression of BAG-1S protein is higher significantly in the colorectal carcinoma than in the colorectal adenoma(p<0.05), the expression of BAG-1S protein is higher significantly in the colorectal adenoma than in the normal colorectal mucosa (p<0.05); the expression of BAG-1S protein is higher significantly in the poorly differentiated colorectal carcinoma than in the well differentiated and the moderately differentiated colorectal carcinoma(p<0.05).Conclusions(1)The positive expression rate of BAG-1 in colorectal adeno carcinoma tissues is significant higher than in normal colorectal mucosa; The positive expression rate of BAG-1 protein in poorly differentiated colorectal carcinoma, in colorectal carcinoma with lymph node metastasis, in Duke's C+D is higher significantly than the positive expression rate of BAG-1 protein in moderately and well differentiated colorectal carcinoma, in colorectal carcinoma without lymph node metastasis, in Duke's A+B. So BAG-1 is associated with the occurance and development of colorectal carcinoma.(2)The isoforms of BAG-1expressed in the colorectal carcinoma is BAG-1S. BAG-1S is associated with differentiation of the colorectal carcinoma.(3)The positive expression rate of VEGF in colorectal adeno carcinoma tissues is significant higher than in normal colorectal mucosa; The positive expression rate of VEGF protein in poorly differentiated colorectal carcinoma, in colorectal carcinoma with full thickness infiltrated, in colorectal carcinoma with lymph node metastasis, in Duke's C+D is higher significantly than the positive expression rate of VEGF protein in moderately and well differentiated colorectal carcinoma, in colorectal carcinoma with only muscular layer infiltrated, in colorectal carcinoma without lymph node metastasis, in Duke'sA+B. So VEGF is associated with the occurance and development of colorectal carcinoma.(4) The expression of BAG-1 and VEGF in colorectal carcinoma is positive correlated.