A Study On The Levels Of CRP And S100B In Rats After Kainic Acid-Induced Seizures

Objective To analysis the correlation between inflammatory indicator CRP and brain injury marker S100B in the rat kainic acid model of temporal lobe epilepsy and then study the role of inflammatory reaction in the brain injury of the temporal lobe epilepsy.Methods 48 male SD rats involved in this study were divided into two groups, the experimental group (40) and the control group (8). Intracerebroventricular injection with 1μg KA was performed in the experimental group and an equal volume of saline was conducted the control group. At 5 different time points—6, 12, 24, 72 hours and 1 week after seizures, the levels of CRP and S100B protein in both serum and CSF of all rats were detected by ELISA, and neuroglias in hippocampal CA3 area were stained with anti-S100B antibody by immunohistochemistry. The relationship between the CRP levels and S100B protein levels was analyzed.Results Levels of CRP and S100B protein in both serum and CSF were significantly higher in the experimental group than those in the control group.In the experimental group, levels of CRP and S100B protein in both serum and CSF increased gradually 6 hours after seizures and declined gradually after reaching their highest level at the 24 hour time point, the change curve of the CRP level was consistent with that of the S100B protein level. At the 24 and 72 hour time points, the number of S100B-immunopositive neuroglia in hippocampal CA3 area increased significantly. There was also an increase in CRP concentration in blood and CSF of rats at the same time point.Conclusions Levels of CRP and S100B protein in both serum and CSF increased in the early stages of epilepsy.There was a positive correlation between CRP level and S100B protein level in rats after KA-induced seizures. Acute inflammatory reaction occured in rat brain after seizures, it maybe involve in epilepsy and seizure-induced brain damage. Neuroglia activation played an important role in the mechanisms of brain inflammatory reaction. Early activated neuroglias could release a large amounts of inflammatory cytokines after KA-induced seizures, which affected the occurrence and development of epilepsy and regulated the activity of neurons. High levels of CRP and S100B in the blood or CSF were expected to be used to predict the prognosis of epilepsy.