| Objective: To investigate the HPV infection and its correlation with the LOH and/or MSI on FHIT gene and their significance in VSCC and VCA. Methods: PCR-SSCP was used to detect the LOH/MSI for amplification with D3S1300 and D3S1481 on FHIT gene in VSCC,VCA and normal vulva. PCR was used to detect HPV DNA 6,11,16,18,31,33 in VSCC,VCA and normal vulva. The VCA patients were treated with local excision andα-2b interferon apply topically.Results:①In normal vulva,VCA and VSCC,the LOH/MSI on D3S1300 were 0%,21.4% and 54.2% respectively,there were statistics significant differences between the 3group(χ2 =17.557,P=0.000<0.05),VSCC and VCA(χ2 =7.366,P=0.007<0.017),VSCC and normal vulva(χ2=15.376,P=0.000<0.017),and there were no statistics significant differences between VCA and normal vulva(Fhisher's =0.047>0.017).②In normal vulva and VCA,The LOH/MSI on D3S1481were 0% and 19.0%,there were statistics significant differences between VCA and normal vulva(Fhisher's=0.046<0.05).③In VSCC,the HR-HPV infections were relevant to the LOH/MSI on D3S1300 (the related coefficient was 0.438,P<0.05), LR-HPV were not relevant to the LOH/MSI on D3S1300 (the related coefficient was 0.158,P=0.461>0.05).④In VCA,both the LR-HPV and the HR-HPV infections were not relevant to the LOH/MSI on on D3S1300(the related coefficient were 0.169 and 0.027,P>0.05).⑤In VCA,both the LR-HPV and the HR-HPV infections were not relevant to the LOH/MSI on on D3S1481(the related coefficient were -0.049 and- 0.194,P>0.05).⑥In VCA, the positive rate of HPV from high to low respectively is HPV11 (90.5%, 38/42), HPV6 (88.1%, 37/42), HPV18 (23.8%, 10/42), HPV16 (7.1%, 3/42), HPV33 (2.4%, 1/42), HPV31 (0%, 0/42).⑦In VSCC, the positive rate of HPV from high to low respectively is HPV11 (79.2%, 19/24), HPV18 (25.0% compared, 6/24), HPV33 (16.7%, 4/24), HPV16 and HPV31 (both 12.5%, 3/24), HPV6 (8.45%, 2/24).⑧In normal vulva,VCA and VSCC,the positive rate of LR-HPV were 10.0%,90.5% and 87.5% respectively,there were statistics significant differences between the three group(χ2 =46.988,P<0.001), normal vulva and VCA(χ2=38.329,P<0.001),the normal vulva and VSCC(χ2=26.263,P<0.001),and there were no statistics significant differences between VCA and VSCC(Fhisher's=0.699>0.017).⑨In normal vulva,VCA and VSCC, the positive rate of HR-HPV were 0%,30.9% and 41.7% respectively, there were statistics significant differences between the three group(χ2 =10.409,P=0.005<0.05),normal vulva and VCA(Fhisher's=0.006<0.017),normal vulva and VSCC (Fhisher's=0.00<0.017), and there were no statistics significant differences between VCA and VSCC(χ2 =0.772,P=0.380>0.017).⑩The recurrence rate of low risk-HPV group(only HPV6 and/or HPV11 infection ) and high risk-HPV group(HPV16 and/or HPV18 and/or HPV31 and/or HPV33 infection ) is 27.6% and 38.5%,there were no statistics significant differences between the two group(Fhisher's=0.495).The recurrence rate of CIN (the 3 patients with pathological changes CIN in the high risk-HPV group) is 3/3.Conclusion:①The LOH/MSI on FHIT gene were possibly relevant to the development and progression of VSCC.②HR-HPV infection had relationship with LOH/MSI on FHIT gene in VSCC.③There were LR-HPV and HR-HPV coinfection in both VSCC and VCA.④In VCA,There had no evidence that HR-HPV infections were more easy to relapses. The recurrence rate were high in HR-HPV infections with pathological changes CIN. |
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