Expression And Signficance Of FHIT And P21 Gene In Vulvar Cancer,Vulvar Intraepithelial Neoplasia And Vulvar Condyloma Acuinatum

Objective: To probe the expression and significance of Fragile histidine triad ( FHIT) and p21 gene in vulvar cancer,vulvar intraepithelial neoplasia(VIN),vulvar condyloma acuminatum(VCA) and normal vulvar tissue .Method: Immunohistochemistry was used to detect FHIT and p21 expression in49 cases of vulvar cancer, 51 cases of VCA,39 cases of VIN(22 cases ofVINⅠ-Ⅱ,17 cases of VINⅢ) and 20 cases of normal vulvar tissue.Resuts:①The positive rate of FHIT in normal vulvar tissue,VINⅠ-Ⅱ,VINⅢand vulvar cancer were 100.0%(20/20),63.6%(14/22),11.8%(2/17),4.1%(2/49),there was statistic significance in the positive rate of FHIT expression between them(χ2 =69.526,P =0.000). the positive rate of FHIT in VINⅠ-Ⅱand VINⅢ,VINⅠ-Ⅱand vulvar cancer has statistic significance (χ2=10.665,P=0.001;χ2=30.848,P=0.000); there was no statistic significance in the positive rate of FHIT expression between VINⅢand vulvar cancer(χ2=1.309,P =0.253).②The positive rate of FHIT in normal vulvar tissue,VCA,vulvar cancer has has statistic significance(χ2=91.249,P=0.000); there was no statistic significance in the positive rate of FHIT expression between vulvar cancer and VCA(χ2=0.171,P=0.680).③The positive rate of FHIT in normal vulvar tissue,VINⅠ-Ⅱ,VINⅢand VCA has statistic significance(χ2=67.704,P=0.000);the positive rate of FHIT in VINⅠ-Ⅱand VINⅢ,VINⅠ-Ⅱand VCA has statistic significance(χ2=10.665,P=0.001;χ2 =28.697,P=0.000); there was no statistic significance in the positive rate of FHIT expression between VINⅢand vulvar cancer(P = 0.592).④The positive rate of p21 in normal vulvar tissue,VCA and vulvar cancer has statistic significance(χ2=16.888,P=0.000); there was no statistic significance in the positive rate of p21 expression between VCA and vulvar cancer(χ2 = 0.11,P=0.917).⑤The positive rate of p21 in normal vulvar tissue,VIN and VCA has statistic significance(χ2=22.667,P=0.000); the positive rate of p21 in VIN and VCA has statistic significance(χ2=17.746,P=0.000); there was no statistic significance in the positive rate of p21 expression between VIN and normal vulvar(χ2=0.069,P=0.793).⑥There was no statistic significance in the positive rate of FHIT expression between high,medium,low histologic grade of vulvar cancer(P>0.05); there was no statistic significance in the positive rate of p21 expression between high,medium,low histologic grade of vulvar cancer(P>0.05).⑦The abnormal expression of FHIT and p21 protein in vulva cancer were not correlated with the lymph node metastasis of vulvar cancer (P>0.05).⑧FHIT gene expression has no relationship with p21 expression in vulva cancer(P>0.05).Conclusion :①The abnormal expression of FHIT and p21 protein might related to the development and progression in vulvar cancer,VIN and VCA; Some Vulvar condyloma acuminata may be the precancerous lesion of vulvar cancer.②The abnormality of FHIT gene is an early event in the developpment and progression of vulvar cancer, it's not too much related to the histological differentiation and lymph node metastasis of vulva cancer. FHIT might become the molecular biological parameter to detect vulvar cancer.③The FHIT gene might has no relationship with p21 gene in the development and progression of vulvar cancer.