The Research For Mechanism Of Sarcandra Glabra Anti-Radiation Damage To The Parotid Gland

Objective: To observe the effect of anti-radiation damage of Sarcandra glabra to the parotid gland and figure the mechanism of it, and discover the mechanism of damage of the salivary gland.Methods: (1) Model parotid radioactive damage: 45 pigs randomly divided into the placebo, radiotherapy group (R group) and Sarcandra glabra+radiotherapy group (SR group), 15 pigs per group. Illuminating parotid gland issues with theγray DT 15Gy single dose and oral administration with 0.3g/Kg till the end of experiment. Observing and recording the changes of pig's weight and behaviors of daily living, and researching the parotid of pig's which being irradiated after 10days 40days and 90days.(2) Using RT-PCR and Western blotting method to detect Bcl-2,Bax, P53 and Caspase3 mRNA and protein expression of parotid gland of pig's which being irradiated after 10 days 40 days and 90 days.Results: (1) Established the model of parotid radioactive damage intervened by Sarcandra glabra successfully. And the recovery of pigs in SR group were better than R group.(2) The result of RT-PCR and Western blotting showed the same trend: The expression of mRNA and protein of Bax, P53 and Caspase3 in radiotherapy group (R group) and Sarcandra glabra+radiotherapy group (SR group) were higher than placebo, and the R group was higher than SR group (P<0.05); But the expression of Bcl-2 in placebo was higher than SR group, and SR group was higher than R group (P<0.05). The expression of each factor was stable. But it became higher increasingly in R group, and lower after firstly high in SR group. The trend of Bcl-2 took the opposite way in each group.Conclusions:(1) It can model parotid radioactive damage successfully with illuminating parotid gland issues with theγray DT 15Gy single dose;(2) The increased apoptosis might be one of the mechanisms of radioactive damage result from ray. (3)Sarcandra glabra can protect the function of parotid gland very well and the mechanism may be that restrained the excessive apoptosis and facilitated the repair of normal cell.