Effect Of Omega-3 Fish Oil On The Intestinal Mucosal Barrier In Severe Acute Pancreatitis

Objective:To observe the relationship between change of Intestinal mucosal barrierpermeability and expression of D-lactate and intestinal fatty acid binding protein (I-FABP) inexperimental serious acute pancreatitis after total parenteral nutrition with omega-3 fish oilsupplementation.To evaluate the protective effect of omega-3 fish oil supplementation on theearly stage of ntestinal mucosal barrier in serious acute pancreatitis.Methods:48 male wistar rats were randomly divided into 3 groups:sham operatedgroup (So,n=16), fish oil supplementation treatment group (F,n=16)and normal sodiumtreatment group (N,n=16).Serious acute pancreatitis was induced in the last two groupsby retro-injected of 5 % sodium cholate (1mL/kg) into pancreatic ducts for almost oneminutes.Fish oil supplementation(2mL/kg) or normal sodium(2mL/kg) was given intravenousto rats in fish oil supplementation treatment group or normal sodium treatment grouprespectively after modeling.To detect the expression of D-lactate and intestinal fattyacid binding protein (I-FABP).Severity of pancreatitis and intestinal was confirmed bylevel of histopathology score.Results: D-lactate, I-FABP concentrations and histopathology score of intestinaland pancreatitis in F group in 6h were markedly lower than N group ( 0.26±0.05mmol/L vs 0.43±0.12mmol/L; 901±61.5ng/ml vs 1510±72.72ng/ml; 1.85±0.34 vs 2.6±0.32;7.3±0.23 vs 8.6±0.31, all p<0.05).D-lactate, I-FABP concentrations and histopathologyscore of intestinal and pancreatitis in F group in 12h were markedly lower than N group(0.48±0.31mmol/L vs 0.75±0.24mmol/L;1135±82.35ng/ml vs 1763±58.24ng/ml;2.7±0.28 vs 3.2±0.75;8.2±1.28 vs 11.2±1.79, p<0.05).Conclusion: Omega-3 fish-oil can reduce the D-lactate and intestinal fatty acid-bindingprotein(I-FABP) concentrations to protect the rat intestinal mucosal barrier,thereby reducing thepermeability of intestinal mucosal and systemic inflammation.