| Objective：To observe the relationship between change of Intestinalmucosal barrier permeability and expression of diaminesoxidase(DAO) and D-lactate in experimental serious acute pancreatitisafter total intra-intestinal nutrition with dietary fiber complexsupplementation. To evaluate the protective effect of dietary fibercomplex supplementation. On the early stage of intestinal mucosal barrierin serious acute pancreatitisMethods: 60 male wistar rats were randomly divided into 4groups，A group is sham operated groupï¼›The rats in group C receivednon-DFC nutrison，while the rats in group B2 or group B3 receivedsoluble dietary fiber(SDF)and insoluble dietary fiber(IDF)of the EN withdifferent proportion(1：3 Or 2：1，respectively) . To evaluate theintestinal mucosal barrier injury of serious acute pancreatitis by detectingthe activity of Plasma diamines oxidase(DAO) and the expression ofD-lactate and observing the extent of the intestinal tissue injury changesin the light microscope.Results: Animal in each group after modeling 12h, the D-lactateconcentrations differences can not believe that there are statistically significant(P>0．05) .D-lactate concentrations in B2 group and B3 group in24h and 48h were markedly lower than B1 group (P<0．05). Thedifferences between B2 group and B3 group can not believe that there arestatistically significant(P>0．05)Animal in each group after modeling 12h, the activity of Plasmadiamines oxidase(DAO) differences can not believe that there arestatistically significant(P>0．05) .Compared with group B1, the levels ofdiamines oxidase of group B2ã€B3were significantly lower (P< 0.05),Lower lever of diamines oxidase and D-lactate appeared in B2 comparedwith B3 (P<0.05)The degree of injury of intestinal mucosal was much lower in groupB2,B3 than group B1in 12h,24h and 48h.Conclusion: dietary fiber complex can reduce the activity of Plasmadiamines oxidase(DAO) and the D-lactate levels and the permeability ofintestinal mucosal to protect the rat intestinal mucosal barrier. |
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