| Objective:To investigate the effects of Lactobacillus acidophilus on the expression of IL-1βand IL-13 in colonic mucosa of DSS-induced rats with Ulcerative Colitis(UC) to find its possible mechanism of treating UC.Methods:UC rat were inducsd by dextran sulfate sodium (DSS), 42 healthy male Sprague-Daw1ey rats were randomly divided into 6 groups:normal,lactobacillus treatment(high-dose),lactobacillus treatment(1ow-dose),positive control(SASP),negative control(NS)and model groups,7 rats were involved in each group.The normal group rates were only fed with distilled water. Each experiment group freely drunk 3% DSS, for 2 days,then 3% DSS was changed to 5% DSS to feed for 2 days, last ,3% DSS for 2 days. There were 7 consecutive days and colitis was induced by SD rats.At the same time, every experimental group was orally administratedNS(0.9%),lactobacillus(1.0x1011CFU/ml),lactobacillus(1.0x1010CFU/ml) ,SASP(67.5mg/ml),once a day individually.The body weight and the character of feces as well as occult blood were observed to know disease activity index of every mouse per day.The eight day later,all rats were sacrificed.The colon length was measured and the colony tissue was handled with HE stained.The expression of IL-1βand IL-13 in the colonic mucosa were detected by immunohistochemical staining.Results:1,Compare with DSS-induced rats model group and NS group general the condition of other treatment groups were better)。SD rats induced with DSS solution and Lactobacillus and SASP decreased DAI scores day by day compared with model group and NS group.Compared with model groupthe colon length of lactobacillus treatment(high-dose)group was statistically significant(P<0.05).In compared with normal group,it had no statistical significance(P>0.05).2,Compared with model group and NS group ,gross injury score in colorectal mucosa and histopathological change of lactobacillus group and SASP group were decreased significantly, there were distinguished differences (p<0.05).3,The IL-lβof DSS group colon mucous is higher than the normal group, the IL-13 is lower than the normal group.Compared with model group and NS group,the levels of IL-1βdecreased in lactobacillus treatmentgroup and SASP group,and the levels of IL-13 was increased in these groups(P<0.01). There's no significant difference between lactobacillus treatment group and SASP group in the levels of IL-1βand the levels of IL-13 in colorectal mucosa of the rats(P>0.05). Conclusion:1,The manifestations of DSS-induced acute colitis rats were similar to clinical symptoms of UC patients. Lactobacillus acidophilus have therapeutical effect to acute UC.2,By increaseing expression of IL-13 and reducing the production of IL-1β,Lactobacillus could control inflammatory response of UC rats .3,There's no significant difference between lactobacillus treatment groups and SASP group in the levels of IL-1βand the levels of IL-13 in colorectal mucosa of the rats,and there could be a similar results in treating UC between lactobacillus and Anti-inflammatory drugs.
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