Compare Research On Pharmacognosy Of Acanthopanax Senticosus Leaf And Acanthopanax Sessiliflorus Leaf

Acanthopanax senticosus(Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus(Rnpr.et Maxim.)Seem, in Araliaceae family, are both precious herbs abundant in the northeastern China. They are precious because the roots, the stems as well as the leaves can be used for medical purposes. However, it has costed damage to these herbs resources to gather roots and stems for medical use. Therefore, it has become a trend currently to develop and use the leaves for medical purposes. Yet, Acanthopanax senticosus(Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem differ not much in morphology, therefore are often mistaken for each other in the practical use.To better use these herbs, this paper compares the leaves of Acanthopanax senticosus(Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem in morphology, chemical components, and antioxidation on the basis of literatures study, so as to provide scientific and reasonable evidences for the development of these two herbs. Also this paper takes hyperin as index to optimize the process, thus provides theoretical basis for future industrialised production.This paper adopts the methods of trait identification and microscopic identification to compare the morphology of the leaves of Acanthopanax senticosus(Rupr.et Maxim.)Harms and Acanthopanax scssiliflorus(Rupr.et Maxim.)Seem, which are accurate and reliable. The results show that:(1)Acanthopanax senticosus(Rupr.et Maxim.)Harms is palmately compound leaves, leaflets usually 5; Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem for the tematcly palmate compound leaves compound, leaflets 3 to 5. (2)Acanthopanax senticosus(Rupr.et Maxim.)Harms epidermis densely brown hair:Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem along the lower epidermis of leaf veins sparsely bristle. (3)Acanthopanax senticosus(Rupr.et Maxim.)Harms leaf margin spiny;Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem leaf margin without spines. (4)Stomata are the infinitive; Acanthopanax senticosus(Rupr.et Maxim.)Harms slightly larger than the upper epidermal cells of leaves Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem epidermal cells. (5)Acanthopanax senticosus(Rupr.et Maxim.)Harms visible cross-section of non-glandular hairs, clusters of calcium oxalate crystals in the palisade tissue, spongy tissue are distributed; in the vascular micro-heart-shaped clock. Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem leaf cross-section don't found non-glandular hairs.clusters of calcium oxalate crystals mainly in the spongy tissue; in the vein of vascular shaped like a hemisphere. (6)Acanthopanax senticosus(Rupr.et Maxim.)Harms leaves no powder characteristics were similar, the main difference is that a large number of non-glandular hairs visible Acanthopanax powder. These characters are very important in differentiation of the leaves of Acanthopanax senlicosus(Rupr.el Maxim.)Harms and Acanthopanax sessiliflorus(Rupr.et Maxim.), and lay a sound foundation for further study of the herbs in Acanthopanax family. This paper adopts HPLC to compare the content of hyperin, syringoside, and chlorogenic acid in the leaves of Accmthopanax seticosus(Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus(Rupr.ct Maxim.)Secm at different collection period. And then analyse the result with SPSS16.0 software. The result shows:in the Acanthopanax senticosus(Rupr.et Maxim.)Harms leaves, the content of hyperin is higher and the content of chlorogenic acid is lower as compared with Acanthopanax sessi liflorus(Rupr.et Maxim.)Seem. But, there is little difference in the contents of syringoside.This paper also adopts ultra violet spectrophotometry to compare the contents of total flavone and total polysaccharide in the leaves of Acanthopanax senticosus (Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus (Rupr.et Maxim.)Seem at different collection period. And then analyse the result with SPSS16.0 software. The result shows:the content of total flavone is higher in the leaves of Acanthopanax senticosus (Rupr.et Maxim.) Harms; while there is a little difference in the content of total polysaccharide. In general, the content of total polysaccharide is higher in the leaves of Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem.This paper adopts atomic absorption spectroscopy to compare the contents of microelements in the leaves of Acanthopanax senticosus (Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus (Rupr.et Maxim.)Seem at different collection period. And then analyse the result with SPSS16.0 software. The results shows:during the entire vegetative cycle of the leaves, the contents of Fe, and Mn, in Acanthopanax sessiliflorus (Rupr.et Maxim.) Seem leaves have been obviously higher. And the content of Zn starts to be higher since 5th, June. These facts demonstrate that from food made of Acanthopanax sessi liflorus(Rupr.et Maxim.)Seem leaves, people can gain more micronutrients, which is helpful for nutrition balance and keeping healthy.This paper adopts DPPH, and the method of hydroxy radical to compare the antioxidation of 75% ethanol extract in the leaves of Acanthopanax senticosus (Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem. The result shows:75% ethanol extract of both leaves can clean DPPH, and hvdroxy radical. And with the increase of the density, the antioxidation of 75% ethanol extract in the leaves increase, showing a good dose-effect relationship. The antioxidation of 75% ethanol extract in the leaves of Acanthopanax senticosus (Rupr.et Maxim.) Harms is stronger, which probably because of the higher content of flavone.This paper adopts the central composite design and response surface method to optimize the process of extracting hyperin from the leaves of Acanthopanax senticosus (Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus (Rupr.et Maxim.)Seem. Taking ethanol volume fraction, extracting time, and menstruum as independent variables, and the extraction rate of hyperin as induced variable; choosing the optimized process through the regression of the complete quadratic response curves surface of independent variables and induced variables, using ridge crest of analysising ethodology. And then predict and analyse. The result shows: the optimized process of hyperin extraction from Acanthopanax senticosus (Rupr.et Maxim.)Harms is:extract 3 times using 59% ethanol under 30-multiple ultrasound,60min each time; while the optimized process of hypcrin extraction from Acanthopanax sessiliflorus(Rupr.et Maxim.)Sccm. is:using 70%cthanol under 23-multiple ultrasound,87 min.In summary, the leaves of Acanthopanax senticasus(Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus (Rupr.et Maxim.)Seem, differs in morphology, major chemical components, and antioxidation. Particularly, they differ greatly in major chemical components. Therefore, they can not be used as substitutes for each other. The hyperin extraction process, chosen through the central composite design and response surface method, easy to practice and accurate, provides basis for industrialised production.Innovation points:1.This paper compares the leaves of Acanthopanax senticosus(Rupr.et Maxim.)Hanns and Acanthopanax sessiliflorus (Rupr.et Maxim.)Seem in morphology for the first time, which provides evidences to distinguish these two herbs from external forms in the future.2.The chemical composition of the leaves of Acanthopanax senticosus (Rupr.et Maxim.) Harms and Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem, this paper optimized the hyperin extraction process, which provides the theoretical basis for future industrialised production.