Extraction Of Ginseng Crude Protein And Research Of Enzymolysis Product

Aiming at the establishment of a feasible basis for utilization of ginseng crude protein extracted from residue, which was from industrial extraction of saponin researches were conducted upon simple extraction methods of Ginseng crude protein and activity of post-enzymolysis product for Free Radical Scavenging.. Crude protein extract of ginseng to the basis of controllable enzyme technology, studies on the enzymatic hydrolysis process, select the best orthogonal analysis of the extraction process. The use of small molecular weight after gel electrophoresis of products of hydrolysis and molecular weight distribution of hydrolysis products for biological activity after a brief discussion. Jilin ginseng industry also discussed the main issues in the current stage of development and problem-solving approach. The main results are as follows:1. Use D-101 type macroporous resin and 001×7 cation exchange resin to extract crude protein, Use D-101 type macroporous resin and 001×7 cation exchange resin to extract crude protein, measured in D-101 type macroporous resin the effective absorption of resin within the resin to add D-101 type macroporous resin of ginseng if the increase will significantly the extraction of crude protein Effect, so that will not affect the extraction of ginsenosides ginseng extract can also be effective crude protein.2. Single factor and orthogonal experiments with crude ginseng discuss the best combination of enzymatic hydrolysis process.The better condition for the papain hydrolysis ginseng protein is:the enzyme concentration 4%, PH 7, temperature55℃, hydrolysis time (t) for 4h. The better condition for the neutral protease hydrolysis ginseng protein is:the enzyme concentration 4%, PH 6, temperature 55℃, hydrolysis time (t) for 3h.3. Low molecular weight gel electropHoresis:protein molecular weight of ginseng extract concentrated in the 70kDa-50kDa and 35kDa-25kDa, between the 35kDa-25kDa protein rich in ginseng. After hydrolysis concentrated in the 70kDa-50kDa bands between the disappearance of,35kDa-25kDa protein content between the decrease in the following turn is shifted to 16kDa, suggesting that the enzymatic hydrolysis of ginseng significantly pHenomenon of protein, and produce small Molecular weight of a mixture of peptides and amino acids4. Through the activity of enzyme solution product research shows:ginseng crude protein hydrolysates on the free radical scavenging, antibacterial, and functional amino acid has a related activity. Neutral protease hydrolyzate (10mg/ml) on DPPH radical scavenging rate 92.50%, papain hydrolyzate(10mg/ml) on DPPH radical scavenging rate 89.91%. Hydroxyl radical, the neutral protease enzyme product (10mg/ml) on hydroxyl radical scavenging rate of 83.17%, papain hydrolyzate (10mg/ml) on hydroxyl radical scavenging rate of 87.61%. lOmg/ml papain inhibitory aspects of hydrolysates rate of 40.248 percent inhibition, and neutral protease inhibitory effect at high concentrations perform well, but at low concentrations (2.5mg/ml) when the inhibition rate To 24.319%. y-aminobutyric acid (GABA) content, the crude protein content of ginseng 7.8%, with the enzyme GABA content in the process of gradual increase in depth, the product of the neutral protease enzyme GABA content of 8.1% papain GABA content of hydrolysates was 8.3%.