Research On Chemical Constituents And Anti-tumor Activity Of The Fruiting Body Of Fomitopsis Officinalis

This paper studied on chemical composition and anti-tumor activity of the fruiting body of Fomitopsis officinalis.On chemistry, ten compoundes were separated from the petroleum ether extraction and EtOAc extract the fruiting body of Fomitopsis officinalis by silica-gel column chromatography and Gel column chromatography.Their purity had been confirmed by thin layer chromatography (TLC),1H-NMR and 13C-NMR, their structures were determined on the basis of their physical and spectral properties (1H-NMR, 13C-NMR,DEPT and MS spectral data) and by comparison of these results to similar data in the literature The ten ompoundes were 4,6,8(14),22(23)-tetraen-3-one-ergostane, Ergosta-7,22,dien-3β-ol, Ergosterol, 3-keto-dehydrosulfurenic acid, dehydroeburicoic acid, Fomefficinic acid A, dehydroeburiconic acid, Fomefficinic acid C, Fomefficinic acid B, Lanosterol-7,11,23-triene-26-acid.The 4,6,8(14),22(23)-tetraen-3-one-ergostane was separated from the fruiting body of Fomitopsis officinalis for the first time.On anti-tumor activity, inhibitory effects of the petroleum ether extraction, the EtOAc fraction, MeOH fraction and four compounds that obtained from EtOAc fraction on the growth of H22 tumor bearing mice were detected. The result indicated that every extraction and compounds all have manifest antitumor effectivity and the inhibition rate was more than 30%, furthermore the EtOAc fraction has the best anti-tumor activity on dose of 1000mg/kg, the rate has reached 64.65% and closed to the positive drug 5-Fu(inhibition rate was 59.98); the inhibition rate of compound 4 (3-keto-dehydrosulfurenic acid) was 75.13%, closed to the positive drug CTX (inhibition rate was 59.98)and highter than positive drug(inhibition rate was 59.98). In addition, all extractions of Fomitopsis officinalis not only can increase the immune organ index, but also can increase the content of IL-2 at different levels, in which the EtOAc fraction of low has significant effects on the thymus index, spleen index and the level of IL-2. From this description, EtOAc fraction and four compounds can effectively inhibit tumor growth, and can improve the body's immune system.In addition, The EtOAc fraction and four compounds that obtained from EtOAc fraction of the fruiting body of Fomitopsis officinalis were studie in vitro anti-tumor activity by MTT method. The results indicated that this four kinds of compounds all have both a certain effect on human liver cancer cells SMMC-7721 and human breast cancer cells MCF-7 in vitro, furthermore the compound 5(dehydroeburicoic acid) has both a certain effect on human liver cancer cells SMMC-7721 and human breast cancer cells MCF-7 in vitro. The max inhibition rate was 83.17% and 85.73% on dose of 500μg/nil.The IC50 rate of compound 5for human human liver cancer cells SMMC-7721 and human breast cancer cells MCF-7 in vitro are respectively 174.25μg/mL and 796.64μg/mL. The second was compound 4 (3-keto-dehydrosulfurenic acid),the max inhibition rate was 74.31% and 83.30% on dose of 500μg/ml.The IC50 rate of compound 4 for human human liver cancer cells SMMC-7721 and human breast cancer cells MCF-7 in vitro are respectively 319.04μg/mL and 62.99μg/mL.Therefore, based on the comprehensive analysis, we can identify that this four kinds of compound were the composition of anti-tumor activity of Fomitopsis officinalis.